GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, firstname.lastname@example.org
Index / MarkerGene / MarkerGene™ Cellular Senescence Assay Kit, Can be used to monitor and detect β-galactosidase activity in senescent cells at pH 6.0 using X-Gal, 1 kit / Product Detail : M1389 MarkerGene™ Cellular Senescence Assay Kit, Can be used to monitor and detect β-galactosidase activity in senescent cells at pH 6.0 using X-Gal, 1 kit
#M1389 MarkerGene™ Cellular Senescence Assay Kit, Can be used to monitor and detect β-galactosidase activity in senescent cells at pH 6.0 using X-Gal, 1 kit
Product name : MarkerGene™ Cellular Senescence Assay Kit, Can be used to monitor and detect β-galactosidase activity in senescent cells at pH 6.0 using X-Gal, 1 kit
Catalog number : M1389
Supplier name : MarkerGene
Data sheet: Ask more or other datasheet now !
More Details about
Can be used to monitor and detect β-galactosidase activity in senescent cells at pH 6.0 using X-Gal.
Many primary cell types, particularly fibroblast cells, have a limited capacity to reproduce in cell culture. Even normal cells, derived from fetal, embryonic or newborn tissue, typically undergo between 40 and 60 cell divisions, but then often stop dividing, becoming senescent. When cells become senescent changes in morphology and gene expression patterns occur. In addition, senescence is also accompanied by increases in acidic senescence-associated β-galactosidase (SA-ÃŸ-Gal). This activity can be monitored using the substrate 5-bromo-4-chloro-3-indolyl-β-galactopyranoside (X-Gal) at pH 6 and is a useful enzymatic marker of cellular senescence (SA-β-Gal). While cells entering senescence show this enzymatic activity, immortalized cell types, including tumor or other quiescent cells, are not stained under these conditions. The implications of these methods in such diverse areas as age-related pathology research, tumor analysis and tissue culture maintenance is significant. When β-Gal cleaves the glycosidic linkage in X-Gal, a soluble, colorless indoxyl derivative is produced, which quickly dimerizes and becomes oxidized to produce a bright blue indigo dye precipitate at the site of β-Gal activity. The dimerization and oxidation reaction is facilitated, in this kit, by a developer buffer solution containing ferric and ferrous ions. Senescent cells can be determined by examining the number of blue cells in the total cell population. The kit provides enough reagents and standards for up to 100 assays, using a microtiterplate method, and includes a detailed protocol for use in cultured mammalian cells.
Contact us about this product :
Our team will respond you as soon as possible !
We have also other products like : MarkerGene™ Cellular Senescence Assay Kit, Can be used to monitor and detect β-galactosidase activity in senescent cells at pH 6.0 using X-Gal, 1 kit
52062 Aachen Deutschland
Support Karolina Elandt
Fax: (+49) 241 56 00 47 88
Logistic :0241 40 08 90 86
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
email@example.com | Gentaur
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123
Canada Montreal +15149077481
Ceská republika Praha +420246019719
Finland Helsset +358942419041
Magyarország Budapest +3619980547
US New York+17185132983
WP1004: Kit Receptor Signaling Pathway
WP1121: Kit Receptor Signaling Pathway
WP1341: Kit Receptor Signaling Pathway
WP147: Kit Receptor Signaling Pathway
WP1603: Nicotine Activity on Chromaffin Cells
WP407: Kit Receptor Signaling Pathway
WP548: neural crest development
WP774: Kit Receptor Signaling Pathway
WP886: Kit Receptor Signaling Pathway
WP1001: Peptide GPCRs
WP1058: Senescence and Autophagy
WP1072: GPCRs, Class A Rhodopsin-like
WP109: UDP-Glucose Conversion
WP1117: Peptide GPCRs
WP1174: Senescence and Autophagy
WP1188: GPCRs, Class A Rhodopsin-like
WP121: Colanic Acid Building Blocks Biosynthesis
WP1251: metapathway biotransformation
WP1267: Senescence and Autophagy
WP1305: Senescence and Autophagy
WP131: Peptide GPCRs
WP1338: Peptide GPCRs
WP1378: Senescence and Autophagy
WP1396: Non-odorant GPCRs
WP1422: Sphingolipid Metabolism
Related Genes :
 Chemical, biological, and DNA markers for tracing slaughterhouse effluent.
 Application for lower facial fat reduction and tightening by static type monopolar 1-MHz radio frequency for body contouring.
 Anti-Infective and Anti-Inflammatory Pharmacotherapies.
 The arterial supply of the nipple areola complex (NAC) and its relations: an analysis of angiographic CT imaging for breast pedicle design.
 Advances in anti-viral immune defence: revealing the importance of the IFN JAK/STAT pathway.
 Feasibility of mini-tablets as a flexible drug delivery tool.
 Is there room for luminal-basal urothelial cell population quantification?
 A Question Mark on Thoracoabdominal Aneurysm.
 Multicenter observational study of abobotulinumtoxinA neurotoxin in cervical dystonia: The ANCHOR-CD registry.
 Oxytocin in the postnatal period: Associations with attachment and maternal caregiving.
Enter catalog number :