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Index / Cleaver / Comet Assay Tank for 20 Slides / Product Detail : CSL-COM20 Comet Assay Tank for 20 Slides
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#CSL-COM20 Comet Assay Tank for 20 Slides

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  Price : 475   EUR
539   USD
368   GBP
1995   Zloty
63554   JPY
3663   NOK
3924   SEK
537   CHF

Product name : Comet Assay Tank for 20 Slides

Catalog number : CSL-COM20

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Availability: Yes

Supplier name : Cleaver

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For Single Cell Gel Electrophoresis
■ Minimise exposure to light and reduce
background DNA damage
■ High efficiency cooling for enhanced
resolution

 COMET assay tanks are available in four slide formats to study single cell gel
electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the detection and quantitation of DNA damage in cells. Each tank’s robust construction from ebony acrylic ensures that cells remain free of exposure to background light and DNA damage during electrophoresis, while a cooled central platform provides a convenient surface for slide preparation and control of slide temperature during the assay. Following electrophoresis DNA damage may be measured using the highly recommended Comet Assay IV Software from Perceptive Instruments.
F250 Chiller With PID control ensuring temperature stability within ±0.5°C, the F250 is the perfect recirculating chiller for active slide temperature control. This chiller also benefits from a maximum flow rate of 15 litres per minute and 0.35 bar pressure, while a small footprint and frontal vents allow multiple units to be aligned side-by-side if required. A versatile working temperature range of +5°C to +40°C makes the chiller suitable for use in other applications such as flat-bed isoelectric focusing.

The Comet Assay

Background: First introduced in 1981 to quantify double-stranded DNA breakages in single cells
exposed to γ-irradiation, the Comet Assay (or SCGE) has since been adapted to analyse specific DNA
lesions and repair processes.
Overview: Following genotoxic insult, such as ionizing radiation, the resultant strand breakage of
supercoiled duplex DNA reduces the size of the large genomic DNA from which these strands are
separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a
‘comet’ as its negatively charged broken ends and fragments migrate towards the anode during
electrophoresis.
Method: After exposure to a genotoxic insult cells are suspended within low melting point agarose and
embedded within a thin layer of agarose on a microscope slide. Cellular protein is then removed by lysis in detergent, when DNA is allowed to unwind in alkaline conditions before electrophoresis. The DNA is electrophoresed, stained and then analysed using software such as Perceptive Instruments Comet Assay IV.
Results: Microscopy imaging is used to measure DNA fluorescence upon staining. In DNA damaged
cells the resultant image resembles a ‘comet’ with the cellular DNA separated into a head and tail. The
head is mainly composed of intact genomic DNA, whereas any fragmented or damaged DNA is
concentrated within and towards the tail

See also:

Comet Assay Software

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