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Index / Biovis / TMB, Ultrasensitive; Appearance Liquid / Product Detail : 1215-100 TMB, Ultrasensitive; Appearance Liquid
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Jul
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#1215-100 TMB, Ultrasensitive; Appearance Liquid

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  Price : 170   EUR
193   USD
131   GBP
714   Zloty
22759   JPY
1311   NOK
1405   SEK
192   CHF

Product name : TMB, Ultrasensitive; Appearance Liquid

Catalog number : 1215-100

Quantity: 100 ml

Availability: Yes

Supplier name : Biovis

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More Details about

 

Alternate Name/Synonyms: 3,3',5,5'-Tetramethylbenzidine; TMBUS

Peptide sequence: N/A

Appearance: Liquid

Formulation: 2.08 mMol L⁻¹ and Hydrogen Peroxide, citric acid buffer at pH 3.3. 

CAS Number: 54827-17-7

Molecular Formula: C₁₆H₂₀N₂

Molecular Weight: 240.35

Purity: N/A

Solubility: N/A

Storage Temp.: +4°C

Shipping Conditions: RT

Description: 3,3'5,5'-Tetramethylbenzidine (TMB) has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H₂O₂ or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 10⁴ mo⁻¹1 cm⁻¹) and diimine (E450 nm = 5.9 x 10⁴ mol⁻¹ cm⁻¹) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes.  TMBUS, available from Biovision Inc., is a single component reagent stable at room temperature and not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP usually employed in immunologic assays.

Handling: Protect from air and moisture

 

USAGE: For Research Use Only! Not For Use in Humans.

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