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#ISB1L InstantBlue _ 1L
Product name : InstantBlue _ 1L
Catalog number : ISB1L
Supplier name : Expede
Data sheet: Ask more or other datasheet now !
More Details about
InstantBlue is the fastest ready-to-use, proprietary, Coomassie® stain available and been optimised to give well-defined protein bands in a single step.
Specially formulated for ultra-fast, sensitive and safe detection of proteins, staining takes minutes without the need to wash, fix, microwave or destain.
Other benefits include high sensitivity (as little as 5 ng per band with BSA - See FAQs), low background staining and non-toxic formulation.
- Ultra-fast staining - providing results in 15 minutes or less.
- Single step procedure - no need to wash, fix, microwave or destain. Just take the gel out of the cassette and place in a container with InstantBlue.
- High sensitivity - approximately 5-25ng of protein detected per band.
- Super-Low background staining - Improves overall gel resolution and sensitivity and no possibility of over staining means that you can leave your gel in the stain indefinitely and still be able to read it.
- Quantitative - Same gel-to-gel performance ideal for quantifying protein by densitometry
- Non-toxic - ideal for regular use and disposal.
- Mass Spec Compatible.
InstantBlue™ is a ready-to-use, proprietary Coomassie® stain that is specially
formulated for ultra-fast, sensitive and safe detection of your proteins. Protein
gels can be stained in minutes without the need to wash, fix or destain.
Only proteins are stained resulting in well defined blue bands on a highly
transparent background. The reduction of background interference results in a
better signal to noise ratio and may also have a positive impact on the overall
resolution and sensitivity.
The InstantBlue formulation is non-toxic and does not contain any methanol.
Proteins stained using the InstantBlue stain are also compatible with mass
spectrometry (MS) analysis.
1L reagent, containing Coomassie dye, ethanol, phosphoric acid and solubilizing
agents in water. (Caution: Phosphoric acid is a corrosive liquid.)
Upon receipt store at + 4°C. Discard any reagents that show discoloration or
evidence of microbial contamination. Be sure to keep the bottle capped when not
Recommended Protocol and Notes on Usage
Before Use :
Mix the InstantBlue solution immediately before use by gently inverting the bottle a few
times (do not shake the bottle to mix the solution).
1) Multiple washes prior to staining with InstantBlue are NOT
required or recommended.
2) An alcohol/acetic acid fixing step prior to staining with
InstantBlue is NOT required or recommended.
3) A destaining step post staining is NOT required or
recommended with InstantBlue.
Standard Protocol :
1) After electrophoresis remove the gel from the tank and transfer directly into the
InstantBlue staining solution. Be sure that the gel moves freely in stain to
facilitate diffusion. Typically ~20 ml is needed to cover the gel.
2) Coloured protein bands will start to develop immediately and a suitable intensity
is typically achieved after 15 minutes incubation at room temperature with
3) Photograph your gel when the required intensity has been achieved. Gels can be
kept in staining solution, but ensure that the gel remains covered with liquid.
Close container to reduce evaporation of InstantBlue. Alternatively the gel can
be stored in ultrapure water after staining for 1 hour in InstantBlue.
4) Once used, the staining solution should be discarded and cannot be reused.
InstantBlue is provided as ready-to-use solution and should not be diluted.
Protocol for Gel Drying :
1) Ensure that the gel has been staining for at least 1 hour.
Although protein bands will be visible after a few minutes of incubation in stain,
the staining process is typically fully completed after 1h incubation. Depending
on the type of gel you are using longer incubation may be necessary. Further
processing of the gel prior to completion of the staining process may result in
protein destaining and reduced sensitivity. If this occurs simply restain the gel
by incubating overnight in InstantBlue.
2) Submerse the gel in approximately 100 ml ultrapure water at ~70°C (heat for
30s to 60s in a microwave oven). Incubate for at least 1 hour while gently
rocking. Optionally adsorbent paper or paper towel can be added. Gels can be
incubated overnight in water.
3) Incubate the gel in a ‘gel drying solution’ (e.g. 4% glycerol, 20% ethanol in
water) for 2 minutes. Incubation of any Coomassie®-stained gel in an alcohol
solution will eventually result in destaining of the bands so avoid incubation for
longer than 5 minutes.
4) The gel is now ready for drying between wetted cellophane membranes.
Protocol for Destaining Protein Bands for MS analysis :
1) Excise the protein band of interest and transfer to a clean Eppendorf tube.
2) Add 1 ml of 30% ethanol or 30% acetone or 30% acetic acid
(Note: Acetic acid may result in acetylation of the N-terminus)
3) Incubate for 20min (incubate at 60°C – 70°C to increase the rate of destaining)
4) Decant supernatant and repeat step 2&3 at least 3 times or until gel is clear
For more detailed protocols please contact your MS facility.
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