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Index / Expede / 3C_Express _ 5,000 Units in 2,500 ul / Product Detail : 3CE0050 3C_Express _ 5,000 Units in 2,500 ul
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Aug
27th

#3CE0050 3C_Express _ 5,000 Units in 2,500 ul

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  Price : 1606   EUR
1822   USD
1245   GBP
6745   Zloty
214853   JPY
12383   NOK
13268   SEK
1816   CHF

Product name : 3C_Express _ 5,000 Units in 2,500 ul

Catalog number : 3CE0050

Quantity:

Availability: Yes

Supplier name : Expede

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More Details about

3C-Express is a recombinant, restriction grade, HRV3C (Human rhinovirus 3C) protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the PreScission Site, and cleaves between Gln and Gly.  3C-Express has robust activity at 4°C and high specific activity. 3C-Express is a 47 kDa protein with both GST and His tags for easy removal using either Amintra NiHIS or Glutathione purification resins

Gel:  A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with 3C-Express (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa.

 

Specific Activity

1 Unit of 3C-Express cleaves >95% of 100 mg of target protein at 4°C for 16 hours.

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