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Index / Nacala / Signal Enhancer HIKARI for Western Blotting and ELISA Solution A / Product Detail : 02272-74 Signal Enhancer HIKARI for Western Blotting and ELISA Solution A
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Dec
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#02272-74 Signal Enhancer HIKARI for Western Blotting and ELISA Solution A

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  Price : 287   EUR
326   USD
223   GBP
1208   Zloty
38504   JPY
2219   NOK
2377   SEK
325   CHF

Product name : Signal Enhancer HIKARI for Western Blotting and ELISA Solution A

Catalog number : 02272-74

Quantity: 250ML

Availability: Yes

Supplier name : Nacala

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About this Product :

Signal Enhancer HIKARI for Western Blotting and ELISA Solution A
http://biotech-map.com/aa_search/table_sample.html?q=ELISA%20kit&filter=
Storage and handling
The storage of the Signal Enhancer HIKARI for Western Blotting and ELISA Solution A has to be done at temperatures two-eight degrees Celsius. It is recommended to avoid 'freezing- thawing' cycles in manner to preserve the kit's sensitivity.

More Details about

Dilute your antibodies with Signal Enhancer HIKARI instead of conventional diluents such as PBS or TBS before performing your next Western blot detection protocol and witness a remarkable increase in the ability to detect the protein of interest and to eliminate undesired background.
Signal Enhancer HIKARI was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as Western blotting and ELISA.

Detection enhancement of Rac1 with treatment of HIKARI in Western blotting

Treatment with Signal Enhancer HIKARI resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal.

Application_1_Rac1

Primary Ab: Anti-Rac1 (1: 1,000) 
Secondary Ab: Anti-Mouse IgG-HRP (1: 2,500) Sample: Rat cortical primary neuron
Detection: ECL

Data courtesy of Dr. K. Ishizuka, Department of Neuroscience, Johns Hopkins University School of Medicine

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Detection enhancement of pJNK with treatment of HIKARI in Western blotting

Treatment with Signal Enhancer HIKARI resulted in clear signal enhancement and background suppression compared with the conventional method using TBS-T.

Application_2_pJNK

Primary Ab: Anti-pJNK (1: 1,000)
Secondary Ab: Anti-rabbit IgG - HRP (1: 5,000)
Sample: Mouse Embryonic Fibroblast (MEF)
Detection: SuperSignal West Pico

Data courtesy of Dr. S. Matsuzawa, 
Signal Transduction, NCI Cancer Center,
Burnham Institute for Medical Research

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References

  1. Feng-Ming Yang et al. FEBS 276, 425-436(2009)
  2. Jian-Bin Wang et al. The Journal of Cell Science 122(12), 2024-2033(2009)
  3. Chunwei Huang et al. Reproductive Toxicology 27, 103-110(2009)
  4. Sawako Yamashiro et al. The Journal of Cell Science 121(Pt 23), 3867-3877(2008)

 

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