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Index / Gentaur / 8_OHdG ELISA  / Product Detail : 50-KOG-200SE 8_OHdG ELISA 
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Sep
25th

#50-KOG-200SE 8_OHdG ELISA 

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  Price : 959   EUR
1089   USD
744   GBP
4031   Zloty
128396   JPY
7400   NOK
7929   SEK
1085   CHF

Product name : 8_OHdG ELISA 

Catalog number : 50-KOG-200SE

Quantity: Stress            96 wells

Availability: Yes

Supplier name : Gentaur

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About this Product :

8_OHdG ELISA 
http://antibody-antibodies.com/anti-elisa/ELISA.html
EIA kit description
8_OHdG ELISA  is an ELISA kit which is manufactured by highest quality antibodies and plates to provide you with excellent and reproducible results in your work. The specifically designed buffers will ensure optimal conditions in each step from dilution of the samples, through the incubation to the blocking and washing.

More Details about

 

 

New 8-OHdG Check ELISA kit

 

 

Catalog number: KOG-200SE

  

Suitable for assessment of oxidative stress using urine samples. For research use only.

 

About 8-hydroxy-2'-deoxyguanosine (8-OHdG):

8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterials from human and animals.

This product is a 8-OHdG ELISA kit utilizing which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. New 8-OHdG Check ELISA is suitable for urine and serum sample from animal and human.

 

Specifications

Assay principle:

Competitive ELISA (detection: 450 nm)

Specifity:

Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).

Measuring range:

0.5 to 200 ng/mL

Format:

96 wells (18 samples in triple assays)

Applications:

Urine from human and animals. 
 

Storage:

Store at 4 - 10°C (don't freeze).

Expiry:

12 months after the day of manufacturing.

Required but not provided:

Micropipet and chip (100 micro L, 1000 micro L).
Measuring pipet (10 mL, 20 mL)/ measuring cylinders.
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
Microplate reader (filter; 450 nm).

 

Content of this kit

8-OHdG Microtiter Plate:

Precoated with 8-OHdG(12 X 8wells, split type)*

1 plate

Primary Antibody:

Anti 8-OHdG antibody, powder.

1 vial

Primary Antibody Solution

 

1 vial (6mL)

Secondary Antibody:

HRP-anti mouse antibody, powder.

1 vial

Secondary Antibody Solution:

 

1 vial (12mL)

Chromatic Solution:

3,3',5,5'-tetramethylbenzidine

1 vial (0.25mL)

Diluting Solution:

H2O2 containing buffer.

1 vial (12mL)

Washing Solution(5x):

 

2 vials (26mL x 2)*

Reaction Terminating Solution:

1M Phosphoric acid.

1 vial (12mL)

Standard 8-OHdG Solution:

Purified 8-OHdG (0.5, 2, 8, 20, 80, 200 ng/mL)

1 vial each

Plate Seal:

 

2 sheets

*:Trial package (KOG-200TE) contains 32 wells (4 X 8 wells), and one vial of Washing solution(5X).

References

1)

H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986)
[ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ]

2)

R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991)
[ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ]

3)

S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa: Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997)
[ Specifity of the antibody clone N45.1.]

4)

Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K: Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA. Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000)
[ Development and assessment of 8-OHdG Check ELISA. ]

5)

Nagayoshi Y, Kawano H, Hokamaki J, Miyamoto S, Kojima S, Shimomura H, Tsujita K, Sakamoto T, Yoshimura M, Ogawa H: Urinary 8-hydroxy-2'-deoxyguanosine levels increase after reperfusion in acute myocardial infarction and may predict subsequent cardiac events. Am J Cardiol. 95(4), p514-517 (2005)
[ Measurement of 8-OHdG in human urine. ]

6)

Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas MJ, Lopez FJ, Abellan P, Villegas JA, Gonzalez-Gallego J: The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. Eur J Appl Physiol 95(5-6),p543-549(2005)
[ Study about exercise, antioxidant supplements and urinary 8-OHdG. ]

7)

Someya T, Kaneko K, Yamada T, Yamashiro Y: Effect of a novel free radical scavenger, edaravone, on puromycin aminonucleoside induced nephrosis in rats. Pediatr Nephrol 20(10),p1430-1434(2005)
[ Effect of antioxidants to 8-OHdG in rat urine. ]

8)

Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K: Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage. J Neurol Sci 250(1-2),p159-161(2006)
[ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ]

 

 

Product name

Code

Assay range

Format

Application

New 8-OHdG Check

KOG-200SE

0.5-200 ng/mL

96 wells

Urine and animal serum.

New 8-OHdG Check Trial Package

KOG-200TE

0.5-200 ng/mL

32 wells

 

      

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