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Index / Trevigen / Mismatched Uracil Glycosylase Enzyme_Buffer / Product Detail : 4125-100-EB Mismatched Uracil Glycosylase Enzyme_Buffer
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Sep
26th

#4125-100-EB Mismatched Uracil Glycosylase Enzyme_Buffer

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  Price : 419   EUR
476   USD
325   GBP
1761   Zloty
56110   JPY
3234   NOK
3465   SEK
474   CHF

Product name : Mismatched Uracil Glycosylase Enzyme_Buffer

Catalog number : 4125-100-EB

Quantity: 100 units

Availability: Yes

Supplier name : Trevigen

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More Details about

Description: E. coli Mug is an 18 kD constitutively expressed protein. The Mug protein can remove a uracil base from within a U:G mismatch as well as act on 3, N4-ethenocytosine-G mismatches (eC:G).
Source: Purified from E. coli containing a recombinant plasmid encoding the E. coli Mug protein.
Unit Definition: One Unit cleaves 1 pmole of a labeled oligonucleotide probe containing 3,N4-ethenocytosine within an oligonucleotide duplex in one hour at 37°C.
Specificity: E. coli Mug catalyzes the excision of the following forms of DNA damage: 3,N4-ethenocytosine in double or single stranded oligonucleotides. It also excises Uracil in Uracil-Guanine mismatches only in double stranded oligonucleotides.
Assay Conditions: 1X REC Buffer 6 (20 mM Tris-Cl (pH 8.0), 0.1 mg/ml BSA, 1 mM EDTA, and 1 mM DTT), 4 pmole of labeled 3,N4-ethenocytosine oligonucleotide annealed to the complement oligonucleotide, and serial dilutions of enzyme in a reaction volume of 20 μl are incubated for 1 hour at 37°C.. For analysis, 20 μl of 2X Loading Buffer (20 mM EDTA, 95% formamide, and 0.13% bromophenol blue) are added and the samples heated to 95°C for 10 min then fast cooled to 4°C. The cleavage products are resolved by 20% denaturing polyacrylamide gel electrophoresis, and percent cleavage quantified.
Storage Buffer: 20 mM Tris-Cl (pH 8.0), 2 mM EDTA, 2.5 mM 2-mercaptoethanol, 1 mM PMSF and 50% (v/v) glycerol.
Storage Conditions: Store at -20°C in a manual defrost freezer.

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