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Index / Trevigen / CometAssay Electrophoresis Starter Kit plus Power Supply for Austrailia / Product Detail : 4250-050-ESK-PS5 CometAssay Electrophoresis Starter Kit plus Power Supply for Austrailia
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#4250-050-ESK-PS5 CometAssay Electrophoresis Starter Kit plus Power Supply for Austrailia

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  Price : 2632   EUR
2987   USD
2041   GBP
11055   Zloty
352124   JPY
20295   NOK
21746   SEK
2976   CHF

Product name : CometAssay Electrophoresis Starter Kit plus Power Supply for Austrailia

Catalog number : 4250-050-ESK-PS5

Quantity:

Availability: Yes

Supplier name : Trevigen

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

Trevigen will no longer be including SYBR® Green (4250-050-05)  in the CometAssay® Kits or offer it for individual purchase effective September 4th, 2012.

 

You may want to check other SYBR Green that Gentaur has in its offer:

20010 SYBR Green I Gel Staining Solution, 10000x 1 mL  
20010-2 SYBR Green I Gel Staining Solution, 10000x 2 x 1 mL  
30010-3 SYBR Green I Gel Staining Solution, 10000x 3 x 1.5 mL  
30010-5 SYBR Green I Gel Staining Solution, 10000x 5 x 1.5 mL

 

4250-050-ESK CometAssay® 2 Well ES Unit w/ Starter Kit 1 system, 1 starter kit, 1 control cell set

COMETASSAY® ELECTROPHORESIS SYSTEM STARTER KIT

Trevigen makes available a complete assay system which includes CometAssay® kits,CometSlides®, Comet Assay® control cells and a specialized electrophoresis unit for alkaline and neutral comet assays.

STARTER KIT (ESK) COMPONENTS

Catalog # Component

  • 4250-05-ES CometAssay® Electrophoresis System
  • 4250-050-K CometAssay® Kit (2 well)
  • 4256-019-CC CometAssay® Control Cells for alkaline Comet or 4257-010-CC for Neutral CometAssay® Control Cells
 
CometAssay® Electrophoresis System
Catalog #: 4250-050-ES

FEATURES:

  • Maintains constant buffer temperature with cooling water chamber and tempered glass platform.
  • Maintains optimal buffer level for consistent results with overlay.
  • Specially designed trays accommodate 2, 20 and 96 well slides and maintain correct position during electrophoresis.
  • Optimized for use with CometAssay Kits and CometAssay Control Cells.
  • Available with area specific power supply.

Trevigen's CometAssay® Electrophoresis System enables investigators to consistently optimize alkaline and neutral comet assays for highly reproducible results, and to standardize electrophoresis methods between individual users and laboratories. Comet assay results can be variable depending on temperature, distance between electrodes, and buffer height. Trevigen has solved these problems by developing a specialized electrophoresis unit.

 

CometAssay® Kit 50 Samples ( 25X2 well slides)
Catalog #: 4250-050-K

APPLICATIONS:                                                                      

  • Applicable for the analysis of either single strand or double strand DNA breaks                                                                                
  • Study cellular responses to DNA damage
  • Screen for inhibitors of DNA repair
  • Test chemicals for toxicity
  • Screen for environmental mutagens
  • Probe for specific types of DNA damage using DNA repair glycosylases

Trevigen's CometAssay®, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA "comet" tail shape and migration pattern allows for assessment of DNA damage. The Neutral CometAssay® is typically used to detect double-stranded breaks, whereas the Alkaline CometAssay® is more sensitive, and is used to detect smaller amounts of damage includingsingle and double-stranded breaks.

ASSAY DESIGN FOR ALKALINE COMET:

Step 1: Cells mixed with low melting agarose are immobilized on specially treated slides and lysed.

  • Membranes and histones removed from the DNA of individually isolated cells.

Step 2: Samples are treated with alkali to unwind and denature the DNA.

  • For detection of single and double-stranded breaks.

Step3: Following alkaline electrophoresis slides are stained and visualized by fluorescent microscopy.

  • Increase in DNA damage results in smaller fragments migrating faster in an electric field.
  • Quantitative measurements of DNA damage

COMPONENTS:

Catalog # Description
4250-050-01 Lysis Solution
4250-050-02 Comet LMAgarose
4250-050-03 CometSlide™ (2 well)
4250-050-04 200 mM EDTA
4250-050-05 SYBR® Green

CometAssay® Alkaline Control Cells
Catalog #: 4256-010-CC

CometAssay® Control Cells for Alkaline Comet Assay

Healthy control cell population (CC0) treated to increase the amount of single-strand damage in populations CC1, CC2 and CC3, respectively.

FEATURES:

  • Provides a range of DNA damage for comparison with statistically distinct populations.
  • Allows for electrophoresis method to be standardized between individual users.
  • Standardized with Trevigen's CometAssay® Electrophoresis System
  • Cell populations remain stable in liquid nitrogen for long term storage.

Trevigen has developed a suspension cell preparation containing different levels of DNA damage to be used as controls with Trevigen's CometAssay® Kits when performing alkaline comet. These cryopreserved controls are designed to act as controls to standardize and compare comet assay results between individual users and laboratories. The AlkalineCometAssay® allows for sensitive detection of both single and double-stranded breaks.

 

Trevigen will no longer be including SYBR® Green (4250-050-05)  in the CometAssay® Kits or offer it for individual purchase effective September 4th, 2012.

 

You may want to check other SYBR Green that Gentaur has in its offer:

20010 SYBR Green I Gel Staining Solution, 10000x 1 mL  
20010-2 SYBR Green I Gel Staining Solution, 10000x 2 x 1 mL  
30010-3 SYBR Green I Gel Staining Solution, 10000x 3 x 1.5 mL  
30010-5 SYBR Green I Gel Staining Solution, 10000x 5 x 1.5 mL

 

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