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Index / ICL / Human IgG / Product Detail : E-80G Human IgG
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Sep
28th

#E-80G Human IgG

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  Price : 497   EUR
564   USD
385   GBP
2089   Zloty
66560   JPY
3836   NOK
4110   SEK
562   CHF

Product name : Human IgG

Catalog number : E-80G

Quantity: 1 x 96 well plate

Availability: Yes

Supplier name : ICL

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More Details about

INTENDED USE
 
The total Human IgG test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG in Human Biological Samples.
 
PRINCIPLE OF THE ASSAY
 
The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
 
Anti-IgG Antibodies Bound To Solid Phase
 │
Standards and Samples Added
IgG*Anti-IgG Complexes Formed
Unbound Sample Proteins Removed
Anti-IgG-HRP Conjugate Added
Anti-IgG-HRP * IgG * Anti-IgG Complexes Formed
Unbound Anti-IgG-HRP Removed
Chromogenic Substrate Added
Determine Bound Enzyme Activity
 
REAGENTS (Quantities sufficient for 96 determinations)
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 L of affinity purified anti-Human IgG antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN IgG ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human IgG.
7. HUMAN IgG CALIBRATOR
One vial containing Human IgG calibrator.
FOR IN VITRO USE ONLY
 
MATERIALS REQUIRED
BUT NOT PROVIDED
  • Precision pipette (2 L to 200L) for making and dispensing dilutions
  • Test tubes
  • Microtitre washer/aspirator
  • Distilled or Deionized H2O
  • Microtitre Plate reader
  • Assorted glassware for the preparation of reagents and buffer solutions
  • Timer
 
 

 

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