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Index / IBT / PiColorlock Gold / Product Detail : 303-0030 PiColorlock Gold
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#303-0030 PiColorlock Gold

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  Price : 218   EUR
247   USD
169   GBP
916   Zloty
29200   JPY
1683   NOK
1803   SEK
246   CHF

Product name : PiColorlock Gold

Catalog number : 303-0030

Quantity: 625/1560 assays

Availability: Yes

Supplier name : IBT

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Key Features of PiColorLock™ - phosphate detection reagent:


Colorimetric Assay – competitor assays are radioactive.
Special additive ensures low backgrounds with acid-labile substrates.
Unparalleled stability of phosphate-dye complexes.
Reagent is compatible with almost any assay buffer.
No inhibition of color development by high concentrations of protein.
Stable reagent formulation - long shelf life.


Introduction

Colorimetric assays for phosphate detection are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.

Competitor assays are beset with several problems including reagent precipitation.  At the heart of Innova Biosciences assay kit is PiColorLock™ Gold a non-radioactive, superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes (green color- see below).

PicolorLock™ Gold has also been designed to:

Prevent background signals arising from non-enzymatic (i.e. acid) hydrolysis of the substrate. The figure below shows ATP incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ gives baseline readings.


Have a large linear range, thus reducing the need for sample dilution.



Related Reagents

PiBind™ resin provides a quick and easy way to remove contaminating Pi from buffers and from samples derived from cells and tissues.

High Throughput Colorimetric ATPase and GTPase assay kits. These non radioactive colorimetric assay kits use a 96 well format and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening.


References:

PiColorlock Gold
Na+ Regulation in the Malaria Parasite Plasmodiumfalciparum Involves the Cation ATPase PfATP4 and Is a Target of the Spiroindolone Antimalarials - Cell Host & Microbe (2013)
A Newly Uncovered Group of Distantly Related Lysine Methyltransferases Preferentially Interact with Molecular Chaperones to Regulate Their Activity - PLoS Genetics (2013)
Polyphosphates inhibit extracellular matrix mineralization in MC3T3-E1 osteoblast cultures - Bone (2013)
Structural and Mechanistic Characterization of L-Histidinol Phosphate Phosphatase from the PHP Family of Proteins - Biochemistry (2013)
Mesenchymal Stem Cell Therapy - Humana Press (2013)
Kinetic Modelling of GlmU Reactions – Prioritization of Reaction for Therapeutic Application - PLoS One (2012)
The unusual mycobacterial chaperonins: evidence for in vivo oligomerization and specialization of function - Molecular Microbiology (2012)
Synthesis and Antibacterial Evaluation of a New Series of N-Alkyl-2-alkynyl/(E)-alkenyl-4-(1H)-quinolones - Molecules (2012)
PPM1H Is a p27 Phosphatase Implicated in Trastuzumab Resistance - Cancer Discovery (2011)
Sepsis impairs alveolar epithelial function by downregulating Na-K-ATPase pump - AJP - Lung Physiol (2011)
Structural and functional insights into DNA-end processing by the archaeal HerA helicase–NurA nuclease complex - Nucleic Acids Research (2011)
Sepsis impairs alveolar epithelial function by downregulating Na-K-ATPase pump - AJP - Lung Physiol (2011)
FXYD Proteins Stabilize Na,K-ATPase, Amplification Of Specific Phosphatidylserine -Protein Interactions - JBC (2011)
Structural and functional insights into DNA-end processing by the archaeal HerA helicase–NurA nuclease complex - Nucl Acids Res (2011)
Stabilization of the α2 isoform of Na,K-ATPase by mutations in a phospholipid binding pocket - JBC (2011)
Characterisation of multiple substrate-specific (d)ITP/(d)XTPase and modelling of deaminated purine nucleotide metabolism - MIMS EPrints (2011)
Substrate uptake, phosphorus repression, and effect of seed culture on glycopeptide antibiotic production: Process model development and experimental validation - Biotechnology and Bioengineering (2010)
Selectivity of Digitalis Glycosides for Isoforms of Human Na,K-ATPase - JBC (2010)
ATP-dependent MurE ligase in Mycobacterium tuberculosis: Biochemical and structural characterisation - Tuberculosis (2010)
Mechanism of Action of an Imidopiperidine Inhibitor of Human Polynucleotide Kinase/Phosphatase - JBC (2010)
Sensor combination and chemometric variable selection for online monitoring of Streptomyces coelicolor fed-batch cultivations - Applied Microbiology and Biotechnology (2010)
Roles of Electrostatics and Conformation in Protein-Crystal Interactions - PLoS One (2010)
Anti-tubercular screening of natural products from Colombian plants: 3-methoxynordomesticine, an inhibitor of MurE ligase of Mycobacterium tuberculosis - J Antimicrob Chemother (2010)
Oligomeric assembly and interactions within the human RuvB-like RuvBL1 and RuvBL2 complexes - Biochem. J. (2010)
Functional study on GTP hydrolysis by the GTP-binding protein from Sulfolobus solfataricus, a member of the HflX family - J. Biochem. (2010)
Functional study on GTP hydrolysis by the GTP-binding protein from Sulfolobus solfataricus, a member of the HflX family - J Biochem (2010)
Identification of a Small Molecule Inhibitor of the Human DNA Repair Enzyme Polynucleotide Kinase/Phosphatase - Cancer Res (2009)
Structural and Mutational Analyses of Deinococcus radiodurans UvrA2 Provide Insight into DNA Binding and Damage Recognition by UvrAs - Structure (2009)
Characterization of Streptococcus gordonii SecA2 as a Paralogue of SecA - J Bacteriol (2009)
Characterization of Human GTPBP3, a GTP-Binding Protein Involved in Mitochondrial tRNA Modification - Mol Cell Biol (2008)
Stabilization of Na+,K+-ATPase Purified from Pichia pastoris Membranes by Specific Interactions with Lipids - Biochemistry (2007)
Purification of the Human α2 Isoform of Na,K-ATPase Expressed in Pichia pastoris. Stabilization by Lipids and FXYD1 - Biochemistry (2007)

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