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#KT-641 Pig Cardiac Troponin-I, High Sensitive ELISA
Product name : Pig Cardiac Troponin-I, High Sensitive ELISA
Catalog number : KT-641
Quantity: 96 tests
Supplier name : Kamiya
Data sheet: Ask more or other datasheet now !
About this Product :Pig Cardiac Troponin-I, High Sensitive ELISA
ELISA Storage and handeling
The storage for all reagents of the Pig Cardiac Troponin-I, High Sensitive ELISA should be done at temperatures between 2 and 8 degrees Celsius. We strongly recommend you to avoid cycles of freezing and thawing in order to preserve the sensitivity of the kit.
More Details about
Pig Cardiac Troponin-I, High Sensitive ELISA is an enzyme immunoassay for the high sensitive
quantitative determination of cardiac troponin-I in pig serum or plasma. For research use only.
Troponin is the contractile regulating protein complex of striated muscle. It consists of three subunits: troponin I, T, and C. Troponin-I exists in three isoforms; one in fast-twitch skeletal muscle, one in slow-twitch skeletal muscle, and one in cardiac muscle. After muscle injury by trauma or ischemia, troponin is released into the bloodstream and its levels correlate well with tissue injury. The sequence of cardiac troponin-I (cTnI) is significantly different from the skeletal muscle isoforms, thus allowing generation of specific anti-cTnI antibodies and development of cTnI specific immunoassays. Because cTnI is uniquely expressed in the heart it provides a specific biomarker of cardiac damage.
Pig Cardiac Troponin-I, High Sensitive ELISA uses two affinity purified antibodies. A rabbit anti-cTnI
polyclonal antibody is used for solid phase immobilization (on the microtiter wells). A goat anti-cTnI peptide-specific
polyclonal antibody is conjugated to horseradish peroxidase (HRP) and used for detection. Samples (serum or plasma) and
calibrators (200 μL) are pipetted into the microtiter wells and incubated for 2 hours on a plate shaker. After washing the wells,
100 μL of diluent and 100 μL of HRP-conjugated anti-cTnI are pipetted into each of the microtiter wells. The plate is incubated for one hour on a plate shaker. During this step, cTnI becomes sandwiched between the solid phase andHRP-conjugated antibodies. The wells are then washed to remove unbound HRP-conjugated antibodies. TMB, an HRP substrate (100 μL), is then added and incubated for 20 minutes, resulting in the development of a blue color. The colordevelopment is stopped by the addition of 1N HCl (100 μL), changing the color to yellow, and absorbance at 450 nm is measured. The concentration of cTnI is proportional to the absorbance at 450 nm and is derived from a calibration curve.
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WP1043: Calcium Regulation in the Cardiac Cell
WP1159: Calcium Regulation in the Cardiac Cell
WP1365: Calcium Regulation in the Cardiac Cell
WP2118: Arrhythmogenic right ventricular cardiomyopathy
WP2406: Cardiac Progenitor Differentiation
WP326: Calcium Regulation in the Cardiac Cell
WP536: Calcium Regulation in the Cardiac Cell
WP553: Calcium Regulation in the Cardiac Cell
WP608: miR-1 in cardiac development
WP808: Calcium Regulation in the Cardiac Cell
WP923: Calcium Regulation in the Cardiac Cell
WP10: IL-9 Signaling Pathway
WP100: Glutathione metabolism
WP1001: Peptide GPCRs
WP1003: Ovarian Infertility Genes
WP1004: Kit Receptor Signaling Pathway
WP1006: metapathway biotransformation
WP1011: T Cell Receptor Signaling Pathway
WP1012: IL-7 Signaling Pathway
WP1017: Type II interferon signaling (IFNG)
WP1019: FAS pathway and Stress induction of HSP regulation
WP1021: Phase I, non P450
WP1022: Hedgehog Signaling Pathway
WP1025: B Cell Receptor Signaling Pathway
Related Genes :
[RACK1 GNB2L1 HLC7 PIG21] Receptor of activated protein C kinase 1 (Cell proliferation-inducing gene 21 protein) (Guanine nucleotide-binding protein subunit beta-2-like 1) (Guanine nucleotide-binding protein subunit beta-like protein 12.3) (Human lung cancer oncogene 7 protein) (HLC-7) (Receptor for activated C kinase) [Cleaved into: Receptor of activated protein C kinase 1, N-terminally processed (Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed)]
[TP53I3 PIG3] Quinone oxidoreductase PIG3 (EC 1.-.-.-) (Tumor protein p53-inducible protein 3) (p53-induced gene 3 protein)
[NEDD4 KIAA0093 NEDD4-1 PIG53] E3 ubiquitin-protein ligase NEDD4 (EC 22.214.171.124) (Cell proliferation-inducing gene 53 protein) (HECT-type E3 ubiquitin transferase NEDD4) (Neural precursor cell expressed developmentally down-regulated protein 4) (NEDD-4)
[KRT18 CYK18 PIG46] Keratin, type I cytoskeletal 18 (Cell proliferation-inducing gene 46 protein) (Cytokeratin-18) (CK-18) (Keratin-18) (K18)
[NCBP2 CBP20 PIG55] Nuclear cap-binding protein subunit 2 (20 kDa nuclear cap-binding protein) (Cell proliferation-inducing gene 55 protein) (NCBP 20 kDa subunit) (CBP20) (NCBP-interacting protein 1) (NIP1)
[GREM1 CKTSF1B1 DAND2 DRM PIG2] Gremlin-1 (Cell proliferation-inducing gene 2 protein) (Cysteine knot superfamily 1, BMP antagonist 1) (DAN domain family member 2) (Down-regulated in Mos-transformed cells protein) (Increased in high glucose protein 2) (IHG-2)
[LITAF PIG7 SIMPLE] Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LPS-induced TNF-alpha factor) (Small integral membrane protein of lysosome/late endosome) (p53-induced gene 7 protein)
[pig-1 W03G1.6] Maternal embryonic leucine zipper kinase (MELK) (EC 126.96.36.199)
[LDHA PIG19] L-lactate dehydrogenase A chain (LDH-A) (EC 188.8.131.52) (Cell proliferation-inducing gene 19 protein) (LDH muscle subunit) (LDH-M) (Renal carcinoma antigen NY-REN-59)
[EPRS GLNS PARS QARS QPRS PIG32] Bifunctional glutamate/proline--tRNA ligase (Bifunctional aminoacyl-tRNA synthetase) (Cell proliferation-inducing gene 32 protein) (Glutamatyl-prolyl-tRNA synthetase) [Includes: Glutamate--tRNA ligase (EC 184.108.40.206) (Glutamyl-tRNA synthetase) (GluRS); Proline--tRNA ligase (EC 220.127.116.11) (Prolyl-tRNA synthetase)]
[UBE2T HSPC150 PIG50] Ubiquitin-conjugating enzyme E2 T (EC 18.104.22.168) (Cell proliferation-inducing gene 50 protein) (E2 ubiquitin-conjugating enzyme T) (Ubiquitin carrier protein T) (Ubiquitin-protein ligase T)
[TGFB1] Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)]
[SERPINF1 PEDF PIG35] Pigment epithelium-derived factor (PEDF) (Cell proliferation-inducing gene 35 protein) (EPC-1) (Serpin F1)
[CAPN1 CANPL1 PIG30] Calpain-1 catalytic subunit (EC 22.214.171.124) (Calcium-activated neutral proteinase 1) (CANP 1) (Calpain mu-type) (Calpain-1 large subunit) (Cell proliferation-inducing gene 30 protein) (Micromolar-calpain) (muCANP)
[PRODH PIG6 POX2] Proline dehydrogenase 1, mitochondrial (EC 126.96.36.199) (Proline oxidase) (Proline oxidase 2) (p53-induced gene 6 protein)
[IMMT HMP MIC60 MINOS2 PIG4 PIG52] MICOS complex subunit MIC60 (Cell proliferation-inducing gene 4/52 protein) (Mitochondrial inner membrane protein) (Mitofilin) (p87/89)
[PTGES MGST1L1 MPGES1 PGES PIG12] Prostaglandin E synthase (EC 188.8.131.52) (Microsomal glutathione S-transferase 1-like 1) (MGST1-L1) (Microsomal prostaglandin E synthase 1) (MPGES-1) (p53-induced gene 12 protein)
[GP] Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
[GPLD1 PIGPLD1] Phosphatidylinositol-glycan-specific phospholipase D (PI-G PLD) (EC 184.108.40.206) (Glycoprotein phospholipase D) (Glycosyl-phosphatidylinositol-specific phospholipase D) (GPI-PLD) (GPI-specific phospholipase D)
[DARS PIG40] Aspartate--tRNA ligase, cytoplasmic (EC 220.127.116.11) (Aspartyl-tRNA synthetase) (AspRS) (Cell proliferation-inducing gene 40 protein)
[GOLGA5 RETII RFG5 PIG31] Golgin subfamily A member 5 (Cell proliferation-inducing gene 31 protein) (Golgin-84) (Protein Ret-II) (RET-fused gene 5 protein)
[MAEA EMP HLC10 PIG5] Macrophage erythroblast attacher (Cell proliferation-inducing gene 5 protein) (Erythroblast macrophage protein) (Human lung cancer oncogene 10 protein) (HLC-10)
[FTH1 FTH FTHL6 OK/SW-cl.84 PIG15] Ferritin heavy chain (Ferritin H subunit) (EC 18.104.22.168) (Cell proliferation-inducing gene 15 protein) [Cleaved into: Ferritin heavy chain, N-terminally processed]
[TNF TNFA TNFSF2] Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF); Intracellular domain 1 (ICD1); Intracellular domain 2 (ICD2); C-domain 1; C-domain 2; Tumor necrosis factor, soluble form]
[PIGA] Phosphatidylinositol N-acetylglucosaminyltransferase subunit A (EC 22.214.171.124) (GlcNAc-PI synthesis protein) (Phosphatidylinositol-glycan biosynthesis class A protein) (PIG-A)
[Ptges iPGES PGES PIG12 rCG_45741] Inducible prostaglandin E synthase (PGE synthase) (PIG12 protein) (Prostaglandin E synthase) (Protein Ptges)
[ENC1 KLHL37 NRPB PIG10] Ectoderm-neural cortex protein 1 (ENC-1) (Kelch-like protein 37) (Nuclear matrix protein NRP/B) (p53-induced gene 10 protein)
[SERPINH1 CBP1 CBP2 HSP47 SERPINH2 PIG14] Serpin H1 (47 kDa heat shock protein) (Arsenic-transactivated protein 3) (AsTP3) (Cell proliferation-inducing gene 14 protein) (Collagen-binding protein) (Colligin) (Rheumatoid arthritis-related antigen RA-A47)
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