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Index / Kamiya / Pig Cardiac Troponin-I, High Sensitive ELISA / Product Detail : KT-641 Pig Cardiac Troponin-I, High Sensitive ELISA
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#KT-641 Pig Cardiac Troponin-I, High Sensitive ELISA

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  Price : 883   EUR
1003   USD
685   GBP
3712   Zloty
118233   JPY
6814   NOK
7301   SEK
999   CHF

Product name : Pig Cardiac Troponin-I, High Sensitive ELISA

Catalog number : KT-641

Quantity: 96 tests

Availability: Yes

Supplier name : Kamiya

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About this Product :

Pig Cardiac Troponin-I, High Sensitive ELISA
ELISA kits expiry date
ELISA kits are stored between 3 and 9 C. The expiry date is mostly affected by the standard. We can supply standards separately if you suspect the storage was at too high temperature or if your lot nr has passed the expiry date and if you still want to work with the same Pig Cardiac Troponin-I, High Sensitive ELISA lot number. For more technical support about $$ contact us at

More Details about


Pig Cardiac Troponin-I, High Sensitive ELISA is an enzyme immunoassay for the high sensitive
quantitative determination of cardiac troponin-I in pig serum or plasma. For research use only.

Troponin is the contractile regulating protein complex of striated muscle. It consists of three subunits: troponin I, T, and C. Troponin-I exists in three isoforms; one in fast-twitch skeletal muscle, one in slow-twitch skeletal muscle, and one in cardiac muscle. After muscle injury by trauma or ischemia, troponin is released into the bloodstream and its levels correlate well with tissue injury. The sequence of cardiac troponin-I (cTnI) is significantly different from the skeletal muscle isoforms, thus allowing generation of specific anti-cTnI antibodies and development of cTnI specific immunoassays. Because cTnI is uniquely expressed in the heart it provides a specific biomarker of cardiac damage.

Pig Cardiac Troponin-I, High Sensitive ELISA uses two affinity purified antibodies. A rabbit anti-cTnI
polyclonal antibody is used for solid phase immobilization (on the microtiter wells). A goat anti-cTnI peptide-specific
polyclonal antibody is conjugated to horseradish peroxidase (HRP) and used for detection. Samples (serum or plasma) and
calibrators (200 μL) are pipetted into the microtiter wells and incubated for 2 hours on a plate shaker. After washing the wells,
100 μL of diluent and 100 μL of HRP-conjugated anti-cTnI are pipetted into each of the microtiter wells. The plate is incubated for one hour on a plate shaker. During this step, cTnI becomes sandwiched between the solid phase andHRP-conjugated antibodies. The wells are then washed to remove unbound HRP-conjugated antibodies. TMB, an HRP substrate (100 μL), is then added and incubated for 20 minutes, resulting in the development of a blue color. The colordevelopment is stopped by the addition of 1N HCl (100 μL), changing the color to yellow, and absorbance at 450 nm is measured. The concentration of cTnI is proportional to the absorbance at 450 nm and is derived from a calibration curve.

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