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#GAM/Fbg/7S goat IgG against mouse fibrinogen
Product name : goat IgG against mouse fibrinogen
Catalog number : GAM/Fbg/7S
Quantity: 10 mg
Supplier name : Nordic Immunological Lab
Data sheet: Ask more or other datasheet now !
About this Product :goat IgG against mouse fibrinogen mus musculus murine goat IgG against mouse fibrinogen detects proteins from variouse species most likely human.
More Details about
Description Purified IgG fraction of polyclonal goat antiserum to mouse fibrinogen
Product code GAM/Fbg/7S
Biological origin Goat
Physical form Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)
Preservative No preservative added, as it may interfere with the antibody activity.
Immunogen Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor
of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts
fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized
by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s
complete adjuvant is used in the first step of the immunization procedure.
Purification The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the
defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis
and double radial immunodiffusion.
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies crossreacting
with other with other plasma proteins. The use of insolubilized adsorption antigens
prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Identity & Specificity The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial
immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse
plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line
obtained with purified fibrinogen. No reaction is obtained with any other plasma protein
component or serum. However, the antiserum may also react with fibrin monomers, circulating
fibrinopeptides and fibrin degradation products.
Cross-reactivity The antiserum does not cross-react with any other component of mouse plasma. Inter-species
cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share
antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
IgG protein concentration 9.8 mg/ml. No foreign proteins added.
Intended use As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting).
When applied in any cytochemical or histochemical procedure or solids phase coupling technique,
the optimum concentration of the IgG preparation should always be established by titration.
Typical working dilutions in histochemistry are usually between 1:50 and 1:250; in ELISA and
comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.
Directions for use The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C;
prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile
distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).
Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage,
this should be removed by centrifugation. It will not affect the performance of the antiserum.
Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Packing Vial with 9.8 mg lyophilized IgG (7S) fraction.
Storage / shelf life Lyophilized at +4°C
reconstituted at or below -20°C
reconstituted at +4°C
at least 10 years
Caution This product should be handled by qualified persons only and appropriate precautions should be
taken in its handling and disposal, and of all associated materials. For in vitro research purposes
NORDIC IMMUNOLOGICAL LABORATORIES
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