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#GAR/C3c goat serum against rabbit C3c
Product name : goat serum against rabbit C3c
Catalog number : GAR/C3c
Quantity: 1 ml
Supplier name : Nordic Immunological Lab
Data sheet: Ask more or other datasheet now !
About this Product :goat serum against rabbit C3c rabbit polyclonal These antibodies are very stable and can be stored up to 2 months at fridge temperature under 10C. Do not freeze taw, rather use goat serum against rabbit C3c from the fridge if your use is less than 1 or 2 weeks.
More Details about
Description Precipitating polyclonal goat antiserum to C3c subunit of rabbit complement C3
Product code GAR/C3c
Biological origin Goat
Physical form Delipidated, heat inactivated, lyophilized, stable whole serum.
Preservative No preservative added.
Immunogen C3 is the most abundant complement protein in rabbit serum. Its biological function strongly resembles that of
C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle; degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response.
The antiserum is raised against C3c, which is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Consequently the antiserum reacts with both native and activated C3. It may also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain. C3c is isolated and purified from pooled normal rabbit serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other rabbit serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Identity & Specificity In immunoelectrophoresis against fresh rabbit serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the ragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of rabbit serum or plasma.
Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of
different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested
Protein concentration Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled goat serum.
No foreign proteins added.
Antibody titre Precipitin titre not less than 1:32 when tested against normal rabbit plasma in agar-block immunodiffusion
Intended use In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3c or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non- precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.
Directions for use In immunoelectrophoresis use 2 μl rabbit plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel.
Packing Vial with 1 ml lyophilized antiserum.
Storage / shelf life Lyophilized at +4°C at least 10 years
reconstituted at or below -20°C 3-5 years
reconstituted at +4°C 7 days
Handling The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be
avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum.
Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Caution This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro research purposes only.
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