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#GIgG/FITC goat IgG, conjugated with FITC
Product name : goat IgG, conjugated with FITC
Catalog number : GIgG/FITC
Quantity: 1 ml
Supplier name : Nordic Immunological Lab
Data sheet: Ask more or other datasheet now !
About this Product :goat IgG, conjugated with FITC FITC conjugates GENTAUR has the longest list of available antibodies ( after Beckton Dickinson)
http://antibody-antibodies.com/anti-elisa/fitc.html for flow cytometry
More Details about
Fluorescein isothiocyanate–conjugated IgG fraction of polyclonal rabbit antiserum to goat
IgG, Fc specific
FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered
saline (PBS, pH 7.2).
No preservative added, as it may interfere with the antibody activity.
Immunogen Purified normal IgG isolated from pooled goat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation
and purification of the IgG fraction by DEAE-chromatography.
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with
other components of the immunoglobulin system or reacting with other serum proteins. The use of
insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in
Identity & Specificity
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal goat plasma and serum, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with
purified IgA, IgM and IgG/Fab fragments.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since IgG of
different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not
been tested in detail.
IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.8.
No foreign proteins added.
Fluorescein isothiocyanate isomer 1. Excitation: 492 nm, emission: 515 nm.
A proprietary technique for the binding to FITC is used, followed by several purification steps to
remove free reactants and protein aggregates. After each step activity and specificity are tested in a
variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.
In direct staining of cytoplasmic IgG in fixed goat cells and tissue substrates; to identify circulating
IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific
antigen or immune complex using a reference antibody of goat in the middle layer of the test
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be
established by titration before being used. Excess labelled antibody must be avoided because it may
cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged
storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to
remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to
use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to
prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated
thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen,
and preferably used the same day. If a slight precipitation occurs upon storage, this should be
removed by centrifugation. It will not affect the performance of the immunoconjugate.
Vial with 1 ml lyophilized immunoconjugate.
Storage / shelf life
Lyophilized at +4°C
reconstituted at or below -20°C
reconstituted at +4°C
at least 10 years
This immunoconjugate should be handled by qualified persons only and appropriate precautions
should be taken in its handling and disposal, and of all associated materials. For in vitro laboratory
research purposes only.
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Related Genes :
[TGF beta] Transforming growth factor beta
[Mboat4 Gm171 Goat] Ghrelin O-acyltransferase (EC 2.3.1.-) (Membrane-bound O-acyltransferase domain-containing protein 4) (O-acyltransferase domain-containing protein 4)
[HMGI-C] HMGI family protein HMGI-C
[LTF] Lactotransferrin (Lactoferrin) (EC 3.4.21.-)
[MBOAT4 GOAT OACT4 FKSG89] Ghrelin O-acyltransferase (EC 2.3.1.-) (Membrane-bound O-acyltransferase domain-containing protein 4) (O-acyltransferase domain-containing protein 4)
[KIT] Mast/stem cell growth factor receptor Kit (SCFR) (EC 188.8.131.52) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (CD antigen CD117)
[CHI3L1] Chitinase-3-like protein 1 (BP40) (Mammary gland protein 40) (MGP-40)
[TNF TNFA TNFSF2] Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF); Intracellular domain 1 (ICD1); Intracellular domain 2 (ICD2); C-domain 1; C-domain 2; Tumor necrosis factor, soluble form]
[LEP OB] Leptin (Obesity factor)
[LALBA] Alpha-lactalbumin (Lactose synthase B protein)
[ND4] NADH-ubiquinone oxidoreductase chain 4 (EC 184.108.40.206) (Fragment)
 Hemoglobin subunit beta-A (Alanine beta-globin) (Beta-A-globin) (Hemoglobin beta-A chain)
[mboat4 goat] Ghrelin O-acyltransferase (EC 2.3.1.-) (Membrane-bound O-acyltransferase domain-containing protein 4)
[CSN3 CSN10 CSNK] Kappa-casein (Kappa-CN)
[Mboat4 Goat] Ghrelin O-acyltransferase (EC 2.3.1.-) (Membrane-bound O-acyltransferase domain-containing protein 4)
[GHRL] Appetite-regulating hormone (Growth hormone secretagogue) (Growth hormone-releasing peptide) (Motilin-related peptide) [Cleaved into: Ghrelin; Obestatin]
[LGB] Beta-lactoglobulin (Beta-LG)
[RNASE1 RNS1] Ribonuclease pancreatic (EC 220.127.116.11) (RNase 1) (RNase A)
[FOXL2] Forkhead box protein L2
[COX1 CO1] Cytochrome c oxidase subunit 1 (EC 18.104.22.168)
[TLR2] Toll-like receptor 2 (CD antigen CD282)
[CSN1S1] Alpha-S1-casein (Alpha-S1-CN)
[HBBC] Hemoglobin subunit beta-C (Beta-C-globin) (Cysteine beta-globin) (Hemoglobin beta-C chain)
[PRNP PRP] Major prion protein (PrP) (CD antigen CD230)
[CXCR3] C-X-C chemokine receptor type 3 (CXC-R3) (CXCR-3) (Interferon-inducible protein 10 receptor) (IP-10 receptor) (CD antigen CD183)
[ITGB2 CD18] Integrin beta-2 (Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta) (Complement receptor C3 subunit beta) (CD antigen CD18)
[CSN1S2] Alpha-S2-casein (Alpha-S2-CN)
[MT-CO1 COI COXI MTCO1] Cytochrome c oxidase subunit 1 (EC 22.214.171.124) (Cytochrome c oxidase polypeptide I)
[psbA] Photosystem II protein D1 (PSII D1 protein) (EC 126.96.36.199) (Photosystem II Q(B) protein)
[HBA1] Hemoglobin subunit alpha-1 (Alpha-1-globin) (Hemoglobin alpha-1 chain)
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