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Index / Nordic Immunological Lab / rabbit serum against human Factor XIII_A / Product Detail : RAHu/FXIII-A rabbit serum against human Factor XIII_A
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Jul
24th

#RAHu/FXIII-A rabbit serum against human Factor XIII_A

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  Price : 310   EUR
352   USD
240   GBP
1303   Zloty
41510   JPY
2392   NOK
2563   SEK
350   CHF

Product name : rabbit serum against human Factor XIII_A

Catalog number : RAHu/FXIII-A

Quantity: 1 ml

Availability: Yes

Supplier name : Nordic Immunological Lab

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About this Product :

rabbit serum against human Factor XIII_A Human samples 80 % of the research is conducted on human samples. GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as rabbit serum against human Factor XIII_A.
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

 

Description
 
Precipitating polyclonal rabbit antiserum to human coagulation factor XIII-A
 
Product code
 
RAHu/FXIII-A
 
Biological origin
 
Rabbit
 
Physical form
 
Delipidated, heat inactivated, lyophilized, stable whole serum.
 
Preservative
 
No preservative added.
 
Immunogen Plasma FXIII is an active transglutaminase until it is activated (FXIIIa). It consists of two A subunits joined as a dimer, connected to two B subunits (A2B2). Each A subunit has a molecular weight of 75,000 and each B subunit of
80,000, making an aggregate molecular weight of the whole molecule of 320,000. Normal plasma contains about
20mg/ml. Platelet FXIII consists of only A subunits synthesized in the megakaryocytes. It accounts for about 50% of
the total blood FXIII activity with a linear relationship between FXIII level and platelet counts.
RAHu/FXIII-A reacts with both plasma and platelet FXIII. Antiserum raised against the B subunit (RAHu/FXIII-S)
reacts exclusively with native and activated FXIIIa in plasma. FXIIIa results from activation of FXIII by thrombin after
the release of fibrinopeptides A from fibrinogen.
Highly purified FXIII-A is used for the immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human
serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or
immune complexes in the antiserum.
Identity & Specificity The defined antibody reactivity is restricted to Factor XIII and FXIIIa in plasma and to platelet FXIII. As tested at the
level of sensitivity of immunoprecipitation techniques a single precipitin line is obtained in bidimensional
electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of
full identity with A subunits of FXIII. No reaction is obtained with FXIII-depleted plasma and with B subunits.
Cross-reactivity The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques.
Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of
different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in
detail.
Protein concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No
foreign proteins added.
Antibody titre Precipitin titre not less than 1:32 when tested against appropriate concentrations of the antigen in agar-block
immunodiffusion titration. The titre is further assayed by quantitative precipitin analysis incubating serial dilutions of
the antiserum with normal plasma and measuring the residual antigen. The amount of Factor XIII precipitated by 1
ml antiserum is about 10 U.
Intended use In precipitating techniques as electroimmunodiffusion, immunoelectrophoresis and single and double radial immunodiffusion
(Mancini, Ouchterlony). To prepare an adsorbent for immunoaffinity purification of FXIII. If used in more
sensitive test procedures or as catching or detection antibody in solid phase immunoassays specificity controls
should always be include.
Increase in plasma FXIII has been observed in type IV hyperlipoproteinaemia and in combined hyperlipodaemia.
Congenital and acquired FXIII deficiencies have also been reported. In some cases of myeloma, inhibition of FXIII
activity by the paraproteins may occur.
Directions for use In immunoelectrophoresis in agarose-plates use 2 μl human plasma or equivalent against 120 μl antiserum. In
double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl
plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion the amount
of antiserum required in the agarose gel is usually between 1 and 2% depending on the test arrangement.
 
Packing
 
Vial with 1 ml lyophilized antiserum.
 
Storage / shelf life Lyophilized at +4°C        at least 10 years
reconstituted at or below -20°C                    3-5 years
reconstituted at +4°C                                      7 days
 
 
 
Handling
 
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or
below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared
by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight
precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the
antiserum.
Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
 
Caution
 
This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its
handling and disposal, and of all associated materials. For in vitro research purposes only.

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