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Index / Panomics / NFkB p52 ELISA Kit / Product Detail : EK3100 NFkB p52 ELISA Kit
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#EK3100 NFkB p52 ELISA Kit

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  Price : 1147   EUR
1301   USD
889   GBP
4817   Zloty
153446   JPY
8844   NOK
9476   SEK
1297   CHF

Product name : NFkB p52 ELISA Kit

Catalog number : EK3100

Quantity: kit

Availability: Yes

Supplier name : Panomics

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About this Product :

NFkB p52 ELISA Kit
Storage and handling
All components of the NFkB p52 ELISA Kit should be stored at temperatures between 2 and 8 degrees Celsius. In order to keep the sensitivity of the kit, please don not freeze and thaw repeatedly.

More Details about

  • Quantify activation of AP-1, E2F-1, EGR1, ERα, FKHR, GATA-1, GR, HIF-1α, HNF3β, MEF-2, MITF,NFκB c-Rel, NFκB p50, NFκB p52, NFκB p65, NFκB Rel-b, p53, PPARα, PPARγ, SMAD-2, SMAD-4, SP-1, and STAT-1
  • Detect activation with as low as 0.5 µg nuclear extract
  • Antibody and double-stranded DNA cis element give assay the best specificity and sensitivity possible
  • Simple workflow: entire assay takes less than 4 hours


The TF Activation ELISA Kits are useful tools to detect and quantify transcription factor activation. The ELISA-based kits combine a fast, user-friendly format with a sensitive and specific assay. The assay detects activation with as low as 0.5 µg of cellular extract and the whole assay is completed in fewer than 3.5 hours.

An EMSA or gel shift assay typically requires 5 µg of nuclear extract and does not have the level of specificity of a TF ELISA which uses an antibody directed to the TF of interest. Each 96-well plate is comprised of individual strips of 8 wells suitable for low or high-throughput applications. These kits may be used to study drug potency, inhibitor or activator proteins, or to probe protein structure and function in signaling pathways.

Nuclear Factor kappa B (NFκB) is a member of the rel family of transcription factors. NFκB plays a key role in the regulation of inflammatory response, apoptosis or tumorigenesis. NFκB is activated by a wide variety of different stimuli such as pro inflammatory cytokines, oxidant free radicals, inhaled particles, ultraviolet radiation and bacterial or viral products. Upon stimulation, several types of kinases belonging to the mitogen-activated protein kinase (MAPK) family cause the phosphorylation and degradation of its Inhibitory kappa B Protein (IκB). The NFκB protein is free from the inhibitor and translocates to the nucleus where it binds to its specific DNA motifs and initiates transcription of genes. NFκB regulates the expression of many genes which play a role in immunity and inflammation e.g. IL2, TNFa, adhesion molecules and acute phase proteins, which are involved in NFκB-mediated responses. The NFκB family consists of a number of structurally related members (p50, p52, RelA, RelB, RelC). These are dimeric (homo and hetero) complexes containing various combinations of these proteins. The members of the NFκB protein group, presently best characterized are the p50/p65 heterodimer and the p50/p50 homodimer complexes.

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