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Index / Panomics / HOXA5 Gene Promoter Reporter Vector / Product Detail : LR1031 HOXA5 Gene Promoter Reporter Vector
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Aug
30th

#LR1031 HOXA5 Gene Promoter Reporter Vector

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  Price : 900   EUR
1022   USD
698   GBP
3783   Zloty
120523   JPY
6946   NOK
7443   SEK
1018   CHF

Product name : HOXA5 Gene Promoter Reporter Vector

Catalog number : LR1031

Quantity: 10 ug

Availability: Yes

Supplier name : Panomics

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More Details about

  • Physiologically relevant - contains cloned promoter region of specific genes
  • Flexible - use for in vivo assays
  • Quantitative - use luciferase activity to measure promoter activity
  • Safe - no radioactivity required
  • Simple - easily applied to high-throughput screening

 

 

The Gene Promoter Reporter Vector is available for 34 different genes. The insert contains approximately 800 bp of 5' flanking region and 50-200 bp of untranslated region of exon 1 of the human genes listed below. This ~1 kb region is located upstream of the start codon of luciferase gene (Panel A.) The vector backbone contains an ampicillin-resistance gene which allows ampicillin selection in E. coli, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production (Panel B).

 

Additional information:

With the Gene Promoter Reporter Vectors, you can quantify the promoter activity of 34 genes. The luciferase assay is a simple, straightforward, and very effective means of measuring promoter response in cells.

 

The Gene Promoter Reporter Vectors in HeLa cells. HeLa cells were transfected with each of the vectors listed and then treated with/without phorbol myristate acetate (PMA). The luciferase activity was significantly enhanced with PMA treatment for TNFα, RB1, and JUNB Gene Promoter Vectors, indicating that these three gene promoters are activated by PMA in HeLa cells, while others are not.

 

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