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Index / Panomics / Procarta Transcription Factor Assay Kit 10 Plex Kit / Product Detail : PC5012 Procarta Transcription Factor Assay Kit 10 Plex Kit
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#PC5012 Procarta Transcription Factor Assay Kit 10 Plex Kit

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  Price : 2516   EUR
2856   USD
1952   GBP
10569   Zloty
336665   JPY
19404   NOK
20791   SEK
2845   CHF

Product name : Procarta Transcription Factor Assay Kit 10 Plex Kit

Catalog number : PC5012

Quantity: Kit

Availability: Yes

Supplier name : Panomics

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More Details about

Transcription factors (TFs), which play crucial roles in the regulation of gene expression in the human genome, are highly regulated by a variety of mechanisms. A single extracellular stimulus can trigger multiple signaling pathways, and these in turn can activate multiple TFs to mediate the inducible expression of target genes. Alterations in the activities of TFs are often associated with human diseases, such as altered activating factor 1, estrogen receptor, and p53 function in cancer, nuclear factor kB in inflammatory diseases, and peroxisome proliferator-activated receptor gamma in obesity. A systematic assay for profiling the activation of TFs will aid in elucidating the mechanisms of TF activation, reveal altered TFs associated with human diseases, and aid in developing assays for drug discovery. We have developed a 40-plex fluorescent microsphere-based TF activation assay system with a 96-well plate format. The assay system enables high-throughput profiling of the DNA binding activity of TFs in multiple samples with high sensitivity.

Procarta Transcription Factor assays combine two technologies: Affymetrix' proprietary detection probe separation method and xMAP® (multi-analyte profiling beads). Together, these two technologies enable the quantitative measurement of DNA binding activity of up to 40 transcription factors (human, mouse or rat) from as little as 500 ng of protein from a whole cell lysate or 250 ng of protein from a nuclear extract. The xMAP system, developed by Luminex Corp., combines flow cytometry, fluorescent-dyed microspheres (beads), lasers and digital signal processing to effectively allow multiplexing of up to 44 unique assays within a single sample.

Monitoring PMA-Mediated Induction in Nuclear Extracts

The Procarta Transcription Factor Assay was used to simultaneously measure the activity of up to 44 TFs in a single assay well. HeLa cells were serum starved overnight and subsequently stimulated PMA or a vehicle control for 4 hours. As expected, PMA induced AP1 and NF-E2 and the activity of NF-1 and STAT4 were unchanged. Sample inductions from the Procarta TF assay were confirmed by EMSA Gel Shift assays. Lane 1 = Free Probe; Lane 2 = HeLa Nuclear Extract; and Lane 3 = PMA Induced HeLa Nuclear Extract.


TF Plex Panel 1

Affymetrix offers 41 different Transcription factors for Panel 1. Users can choose from the 41 TF's below to create their own Plex set.

RUNX/AML ELK1 ISRE OCT
AP1 ER MEF-2 P53
AP2 ETS/PEA MYOD PAX3
AR FAST1 NF-1 PAX5
ATF2 FKHR-1 NFAT PPAR
BRN3 GATA-1 NF-E1(YY1) SMAD
CEBP GR/PR NF-E2 STAT1
C-MYB HIF-1 NFkB* STAT3
CREB HNF1 NKX-2.5 STAT4
E2F1 IRF NF-Y STAT5
TFIID*      

*TFIID and NFkB must be included in all By Request TF Plex Sets.


TF Plex Panel 2

Affymetrix offers 44 different Transcription factors for Panel 2. Users can choose from the 44 TF's below to create their own Plex set.

EVI-1 GAG IKAROS HBS/XBP
HAS+HBS ALF-1/TAL-1 TREF-1/2 EGR
NFkB* c-MYC Neuro-D1 PDX-1
TR MRE ELF-1 SRE
H4TF HiNF KPF-1 LF-A1
LvF MTF PUR-1 RB
TFE-3 AP-1 USF-1 XRE
GFI-1 NPAS2 PIT-1 SRY
E47 CCAAT AP-4 CDP
XBP-1 CEF-1 ANTI-RE COUP-TF
SIE HSF TR (DR-4) TFIID*

*TFIID and NFkB must be included in all By Request TF Plex Sets.

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