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Index / SibEm / Abs I Enzyme CC^TCGAGG PrototypeAbs I / Product Detail : E535 Abs I Enzyme CC^TCGAGG PrototypeAbs I
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#E535 Abs I Enzyme CC^TCGAGG PrototypeAbs I

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  Price : 324   EUR
367   USD
251   GBP
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43371   JPY
2499   NOK
2678   SEK
366   CHF

Product name : Abs I Enzyme CC^TCGAGG PrototypeAbs I

Catalog number : E535

Quantity: 50 Package u.a.

Availability: Yes

Supplier name : SibEm

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More Details about

Name Abs I  
Cat. # E535 
Package: 50
Concentration 500-1000
Recognition site

CC↑TCGAGG
GGAGCT↓CC


Source Arthrobacter species 7M06
Assayed on  pUC19SE/DriI - digest
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19SE/DriI -digest in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer SE-buffer AbsI (10 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 50 mM KCl; 1mM DTT.)
Enzyme activity (%)
B                           G                    O                  W                 Y                  R
75 - 100        10 - 25            0 - 10            50 - 75        0 - 10            50
Optimal temperature
37oC
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA;0,05% Triton X-100; 50% glycerol; Store at -20°C.
Ligations After 2-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme  10 X SE-buffer AbsI
Methylation sensitivity not tested
Inactivation 20 minutes under
65oC
Notes A long incubation time may result in star activity.

References:  V.A. Chernukhin, Yu.G. Kashirina, Yu.E. Tomilova, D.A. Gonchar, V.S. Dedkov, N.A. Mikhnenkova, S.Kh. Degtryarev. New Eight Bases Cutter AbsI from Arthrobacter species Recognizes Palindromic DNA Sequence 5’-CC^TCGAGG-3’ // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 2, pp 29-34, 2006

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