GENTAUR Molecular Products.
Monoclonal Antibody, ELISA Kit, Polyclonal Antibody, Recombinant/Purified Protein.GENTAUR Belgium BVBA BE0473327336
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Index / SibEm / Hae III Enzyme PrototypeHae III / Product Detail : E067 Hae III Enzyme PrototypeHae III
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Apr
21th
#E067 Hae III Enzyme PrototypeHae III
Price : | 80 | EUR |
91 | USD | |
62 | GBP | |
337 | Zloty | |
10735 | JPY | |
618 | NOK | |
663 | SEK | |
90 | CHF |
Product name : Hae III Enzyme PrototypeHae III
Catalog number : E067
Quantity: 2000 Package u.a.
Availability: Yes
Supplier name : SibEm
Data sheet: Ask more or other datasheet now !
More Details about
Recognition site |
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Source | E.coli strain, that carries the cloned Hae III gene from Haemophilus aegyptius | ||||||||||||||
Assayed on | Lambda DNA | ||||||||||||||
Unit | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | ||||||||||||||
Optimal SE-buffer | G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) | ||||||||||||||
Enzyme activity (%) |
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Optimal temperature |
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Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C. | ||||||||||||||
Ligations | After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. | ||||||||||||||
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. | ||||||||||||||
Reagents Supplied with Enzyme | 10 X SE-buffer G | ||||||||||||||
Methylation sensitivity | not tested | ||||||||||||||
Inactivation 20 minutes under |
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MSDS: | Download MSDS as PDF | ||||||||||||||
References: |
Middleton, J.H., Edgell, M.H., Hutchison, C.A. J. Virol. 10:42-50 (1972). ![]() ![]() |
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Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322 |
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Related products : Hae III Enzyme PrototypeHae III
Gentaurpub
Pathways :
WP142: mRNA processing
WP1493: Carbon assimilation C4 pathway
WP2340: Thiamine (vitamin B1) biosynthesis and salvage
WP2341: vitamin B1 (thiamin) biosynthesis and salvage pathway
WP2344: vitamin B6 (pyridoxine, pyridoxal, pyridoxamine) biosynthesis and salvage pathway
WP2349: vitamin B3 (niacin), NAD and NADP biosynthesis pathway
WP626: Abscisic Acid Biosynthesis
Related Genes :
[RLK5 HAE At4g28490 F21O9.180] Receptor-like protein kinase 5 (EC 2.7.10.1) (EC 2.7.11.1) (Protein HAESA)
[SERPING1 C1IN C1NH] Plasma protease C1 inhibitor (C1 Inh) (C1Inh) (C1 esterase inhibitor) (C1-inhibiting factor) (Serpin G1)
[F12] Coagulation factor XII (EC 3.4.21.38) (Hageman factor) (HAF) [Cleaved into: Coagulation factor XIIa heavy chain; Beta-factor XIIa part 1; Coagulation factor XIIa light chain (Beta-factor XIIa part 2)]
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
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