Gentaur Molecular :SibEm \ Hae III Enzyme PrototypeHae III \ E067

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Apr
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#E067 Hae III Enzyme PrototypeHae III

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Price :	80	EUR
	91	USD
	62	GBP
	337	Zloty
	10735	JPY
	618	NOK
	663	SEK
	90	CHF

Product name : Hae III Enzyme PrototypeHae III

Catalog number : E067

Quantity: 2000 Package u.a.

Availability: Yes

Supplier name : SibEm

Data sheet: Ask more or other datasheet now !

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More Details about

Recognition site

GG↑CC
CC↓GG

Source

E.coli strain, that carries the cloned Hae III gene from Haemophilus aegyptius

Assayed on

Lambda DNA

Unit

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Optimal SE-buffer

G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 50 mM NaCl; 1 mM DTT.)

Enzyme activity (%)

B	G	O	W	Y	R
75 - 100	100	25 - 50	50 - 75	50 - 75	100

Optimal temperature

37^oC

Storage conditions

10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.

Ligations

After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis

No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Reagents Supplied with Enzyme

10 X SE-buffer G

Methylation sensitivity

not tested

Inactivation 20 minutes under

80^oC

MSDS:

Download MSDS as PDF

References:

Middleton, J.H., Edgell, M.H., Hutchison, C.A. J. Virol. 10:42-50 (1972).

V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Mammalian chromosomal DNA digestion with restriction endonucleases in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 29-38, 2006

Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram

Fragment lengths

4092, 2366, 2099, 1784, 1310, 984, 933, 917, 890, 771, 740, 696, 643, 633, 612, 560, 547, 534, 533, 527, 525, 521, 506, 493, 482, 476, 469, 460, 453, 452, 450, 446, 440, 437, 433, 422, 414, 407, 405, 397, 394, 362, 352, 351, 350, 346, 343, 334, 328, 324, 312, 307, 297, 287, 281, 272, 265, 264, 261, 259, 257, 235, 234, 228, 223, 222, 221, 217, 213, 212, 211, 209, 208, 184, 181, 178, 175, 171, 168, 161, 158, 156, 145, 142, 135, 131, 130, 127, 111, 110, 105, 104, 103, 102, 100, 97, 96, 93, 91, 85, 84, 83, 82, 79, 66, 65, 64, 62, 60, 57, 56,

LAMBDA DNA


M	1	2	3	4	5	M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322

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