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Index / SibEm / Hae III Enzyme PrototypeHae III / Product Detail : E067 Hae III Enzyme PrototypeHae III
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#E067 Hae III Enzyme PrototypeHae III

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  Price : 80   EUR
91   USD
62   GBP
337   Zloty
10735   JPY
618   NOK
663   SEK
90   CHF

Product name : Hae III Enzyme PrototypeHae III

Catalog number : E067

Quantity: 2000 Package u.a.

Availability: Yes

Supplier name : SibEm

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More Details about

 

Recognition site
GGCC
CCGG
Source E.coli strain, that carries the cloned Hae III gene from Haemophilus aegyptius
Assayed on Lambda DNA
Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
B G O W Y R
75 - 100 100 25 - 50 50 - 75 50 - 75 100
Optimal temperature
37oC
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer G
Methylation sensitivity not tested
Inactivation 20 minutes under
80oC
MSDS: Download MSDS as PDF
References: Middleton, J.H., Edgell, M.H., Hutchison, C.A. J. Virol. 10:42-50 (1972). 
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006 
 M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Mammalian chromosomal DNA digestion with restriction endonucleases in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 29-38, 2006 
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
 
Fragment lengths
 
4092, 2366, 2099, 1784, 1310, 984, 933, 917, 890, 771, 740, 696, 643, 633, 612, 560, 547, 534, 533, 527, 525, 521, 506, 493, 482, 476, 469, 460, 453, 452, 450, 446, 440, 437, 433, 422, 414, 407, 405, 397, 394, 362, 352, 351, 350, 346, 343, 334, 328, 324, 312, 307, 297, 287, 281, 272, 265, 264, 261, 259, 257, 235, 234, 228, 223, 222, 221, 217, 213, 212, 211, 209, 208, 184, 181, 178, 175, 171, 168, 161, 158, 156, 145, 142, 135, 131, 130, 127, 111, 110, 105, 104, 103, 102, 100, 97, 96, 93, 91, 85, 84, 83, 82, 79, 66, 65, 64, 62, 60, 57, 56,
LAMBDA DNA
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M 1 2 3 4 5 M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322

 

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