GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, firstname.lastname@example.org
#DBGD-100 QuantiChrom™ β-Glucosidase Assay Kit
Product name : QuantiChrom™ β-Glucosidase Assay Kit
Catalog number : DBGD-100
Quantity: 100 tests
Supplier name : Bioassays
Data sheet: Ask more or other datasheet now !
About this Product :QuantiChrom™ β-Glucosidase Assay Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ β-Glucosidase Assay Kit.
More Details about
b-GLUCOSIDASE is a glucosidase enzyme which acts upon β1->4 bonds
linking two glucose or glucose-substituted molecules (i.e., the disaccharide
cellobiose). b-Glucosidases are required by organisms (some fungi,
bacteria, termites) for consumption of cellulose. Lysozyme is also a b-
glucosidase and is present in tears to prevent bacterial infection of the eye.
In humans, lower activity of a b-glucosidase isoform (lysosomal glucocerebrosidase)
has been related to Gaucher's disease and Parkinson's
Simple, direct and automation-ready procedures for measuring b-
glucosidase activity are becoming popular in Research and Drug Discovery.
BioAssay Systems' QuantiChromTM b-Glucosidase Assay Kit is designed to
measure b-glucosidase activity directly in biological samples without
pretreatment. The improved method utilizes p-nitrophenyl-b-Dglucopyranoside
that is hydrolyzed specifically by b-glucosidase into a
yellow colored product (maximal absorbance at 405nm). The rate of the
reaction is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Use 20 μL sample. The detection
limit is 2 U/L, linear up to 250 U/L.
Homogeneous and simple procedure. Simple “mix-and-measure”
procedure allows reliable quantitation of b-glucosidase activity within 20
Robust and amenable to HTS. All reagents are compatible with highthroughput
liquid handling instruments.
Direct Assays: b-glucosidase activity in biological samples.
Characterization and Quality Control for b-glucosidase production.
Drug Discovery: high-throughput screen for b-glucosidase modulators.
KIT CONTENTS (100 tests in 96-well plates)
Assay Buffer: 24 mL (pH 7.0)
b-NPG Substrate: 1 mL
Calibrator: 10 mL (equivalent to 250 U/L)
Storage conditions. The kit is shipped at room temperature. Store all
components at -20°C. Shelf life of 6 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents. Please
refer to Material Safety Data Sheet for detailed information.
PROCEDURES. This assay is based on a kinetic reaction. Use of a
multi-channel pipettor is recommended. Addition of Working Reagent to
samples should be quick and mixing should be brief but thorough. Assays
can be executed at room temperature or 37°C.
Reagent preparation: equilibrate reagents to room temperature. The
Working Solution is prepared by mixing for each 96-well assay, 200 μL
Assay Buffer and 8 μL b-NPG substrate (final 1.0 mM). Fresh reconstitution
is recommended, although the Working Solution is stable for at least one
day at room temperature.
Sample preparation: enzyme samples can be in 50 mM phosphate (pH 7.0)
buffer or in any other suitable enzyme buffer. The following chemicals are
known to affect the enzyme activity and should be avoided. SH-containing
reagents (e.g. dithiothreitol, 2-mercaptoethanol, glutathione), Ca2+, Cu2+,
Fe3+/Fe2+, Hg2+, Mg2+, Ni2+, Zn2+, SDS, EDTA and Tris.
Procedure using 96-well plate:
1. Transfer 20 μL distilled water (H2O) to two wells of a clear bottom 96-well
plate. Add 200 μL H2O to one of these wells and 200 μL Calibrator to the
other well (total volume 220 μL).
Transfer 20 μL samples into other wells. Transfer 200 μL Working
Reagent to the sample wells only. The final reaction volume in the
sample wells is 220 μL. Tap plate briefly to mix.
2. Read OD405nm (t = 0), and again after 20 min (t = 20 min) on a plate reader.
4. Calculation: b-glucosidase activity of the sample (U/L) is
OD20 – OD0
ODCALIBRATOR – ODH2O
b-Glucosidase Activity × 250 (U/L)
OD20 and OD0 are OD405nm values of sample at 20 and 0 min,
respectively. ODCALIBRATOR and ODH2O are OD405nm values of Calibrator
and H2O at 20 min.
Unit definition: one unit of enzyme catalyzes the hydrolysis of 1 μmole of
substrate per min at pH 7.0.
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipeting devices and accessories (e.g. multi-channel pipettor). Clear
bottom 96-well plates (e.g. Corning Costar) and plate reader.
Contact us about this product :
Our team will respond you as soon as possible !
52062 Aachen Deutschland
Support Karolina Elandt
Fax: (+49) 241 56 00 47 88
Logistic :0241 40 08 90 86
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
email@example.com | Gentaur
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123
Canada Montreal +15149077481
Ceská republika Praha +420246019719
Finland Helsset +358942419041
Magyarország Budapest +3619980547
US New York+17185132983
WP1004: Kit Receptor Signaling Pathway
WP1121: Kit Receptor Signaling Pathway
WP1341: Kit Receptor Signaling Pathway
WP147: Kit Receptor Signaling Pathway
WP407: Kit Receptor Signaling Pathway
WP548: neural crest development
WP774: Kit Receptor Signaling Pathway
WP886: Kit Receptor Signaling Pathway
Related Genes :
 Gene flow from an adaptively divergent source causes rescue through genetic and demographic factors in two wild populations of Trinidadian guppies.
 Bioresorbable vascular scaffolds technology: current use and future developments.
 Case 22-2016.
 PHARMACOKINETIC EVALUATION OF A LONG-ACTING FENTANYL SOLUTION AFTER TRANSDERMAL ADMINISTRATION IN HELMETED GUINEAFOWL (NUMIDA MELEAGRIS).
 Intrasexual selection favors an immune-correlated color ornament in a dragonfly.
 Strategies to influence RN specialty certification.
 Time to bury "hypertension".
 Reduced LINE-1 methylation is associated with arsenic-induced genotoxic stress in children.
 The Integrin Receptor in Biologically Relevant Bilayers: Insights from Molecular Dynamics Simulations.
 Obesity Disrupts the Rhythmic Profiles of Maternal and Fetal Progesterone in Rat Pregnancy.
Enter catalog number :