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Index / Bioassays / QuantiChrom™ Formaldehyde Assay Kit / Product Detail : DFOR-100 QuantiChrom™ Formaldehyde Assay Kit
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Aug
30th

#DFOR-100 QuantiChrom™ Formaldehyde Assay Kit

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  Price : 363   EUR
412   USD
282   GBP
1527   Zloty
48667   JPY
2805   NOK
3005   SEK
411   CHF

Product name : QuantiChrom™ Formaldehyde Assay Kit

Catalog number : DFOR-100

Quantity: 100 tests

Availability: Yes

Supplier name : Bioassays

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About this Product :

QuantiChrom™ Formaldehyde Assay Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ Formaldehyde Assay Kit.
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DESCRIPTION
FORMALDEHYDE (methanal) is the simplest aldehyde. It is widely
employed in industry for wide range of applications. Formaldehyde is also
used as a disinfectant and is a commonly utilized tissue fixative and
embalming agent. Formaldehyde is naturally present in all tissues and body
fluids. Recently it has been shown that some cancer types exhibit elevated
formaldehyde levels. Increased formaldehyde concentration in urine has
been associated with prostate and bladder cancer. Thus, measuring
formaldehyde in urine can be a very useful tool when studying cancer.
BioAssay Systems’ newly designed Formaldehyde Assay Kit provides a
convenient fluorimetric means to measure formaldehyde in biological
samples. In the assay, formaldehyde is derivatized with acetoacetanilide in
the presence of ammonia. The resulting fluorescent product is then
quantified fluorimetrically (lexc/em = 370/470nm). The assay is simple,
sensitive, stable and high-throughput adaptable. The assay can detect as
low as 1.5 μM formaldehyde in biological samples.
KEY FEATURES
Safe. Non-radioactive assay.
Sensitive and accurate. As low as 1.5 μM Formaldehyde can be
quantified.
Homogeneous and convenient. "Mix-incubate-measure" type assay. No
wash and reagent transfer steps are involved.
Robust and amenable to HTS: Can be readily automated on HTS liquid
handling systems for processing thousands of samples per day.
APPLICATIONS
Formaldehyde determination in urine and other biological samples.
KIT CONTENTS
Reagent A: 5 mL Reagent B: 3 mL Standard: 50 μL
10% TCA: 5 mL Neutralizer: 2 x 1.5 mL
Storage conditions: The kit is shipped at room temperature. Store
Reagent A and Reagent B at 4°C. Store all other reagents at RT. Shelf life
of 18 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents. Please
refer to Material Safety Data Sheet for detailed information.
ASSAY PROCEDURE
Use black flat-bottom plates. Prior to assay, bring all reagents to room
temperature.
1. Standards. First prepare a 35 mM Formaldehyde stock by diluting 4 μL of
the provided Standard with 1490 μL dH2O. Next, dilute 4 μL of the 35
mM Formaldehyde with 1396 μL dH2O to make a 100 μM Premix. Dilute
standard as follows.
No Premix + H2O Formaldehyde (μM)
1 100 μL + 0 μL 100
2 60 μL + 40 μL 60
3 30 μL + 70 μL 30
4 0 μL + 100 μL 0
Transfer 50 μL standards into separate wells of the plate.
2. Sample Preparation. Urine samples should be diluted 2-5 fold with
dH2O. If urine samples contain visible particulates, then the samples
should be cleared by either filtration or centrifugation (14000 rpm, 5 min).
Samples high in protein (cell lysate, serum, etc.) need to be
deproteinated and neutralized prior to assaying. To deproteinate, add
50 μL 10% TCA per 100 μL sample. Vortex and centrifuge for 5 min at
14000 rpm. Transfer 100 μL of clear supernatant to a clean tube and
neutralize with 25 μL Neutralizer. Note: Measured RFU’s for
deproteinated samples need to be multiplied by 1.875 to compensate for
the resulting dilution of the sample.
Samples not measured the same day should be stored frozen,
preferably at -80°C.
3. Add 50 μL of each prepared sample to two separate wells of the plate
(one well will be used as a Sample Blank).
4. Prepare Working Reagent for each standard and sample well by mixing
33 μL Reagent A and 22 μL Reagent B. For the Sample Blanks, make
the following Working Reagent: 33 μL Reagent A + 22 μL dH2O. Add 50
μL of the appropriate Working Reagent to each well. Tap plate to mix.
Incubate at room temperature for 30 min protected from light.
5. Read fluorescence intensity at lexc = 370 nm and lem = 470 nm.
CALCULATION
Plot the RFU measured at 30 min for each standard against the standard
concentrations. Determine the slope using linear regression fitting. The
Formaldehyde concentration of a Sample is calculated as
RFUSAMPLE – RFUBLANK – RFUWATER
Slope
[Formaldehyde] = × n (μM)
where RFUSAMPLE, RFUBLANK and RFUWATER are the measured fluorescence
values of the sample, sample blank and water (std #4) respectively. Slope
is the slope of the standard curve in μM-1 and n is the dilution factor (n =
1.875 for deproteinated samples). Note: if the Sample Formaldehyde
concentration is higher than the 100 μM prior to applying the dilution factor,
dilute sample in water and repeat the assay. Multiply result by the dilution
factor.
Conversion factor: 1 μM formaldehyde is equivalent to 30 ppb.
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipetting devices, centrifuge tubes, black flat bottom 96-well plates (e.g.
Corning Costar) and plate reader.

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