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Index / SibEm / Msp I / Product Detail : E091 Msp I
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  Price : 90   EUR
103   USD
70   GBP
381   Zloty
12166   JPY
701   NOK
751   SEK
102   CHF

Product name : Msp I

Catalog number : E091

Quantity: 1000

Availability: Yes

Supplier name : SibEm

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More Details about

 

Recognition site
CCGG
GGCC
Source Moraxella species
Assayed on Lambda DNA
Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Enzyme activity (%)
B G O W Y R
100 75 - 100 50 - 75 75 - 100 75 - 100 100
Optimal temperature
37oC
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer B
Methylation sensitivity not tested
Inactivation 20 minutes under
65oC

 

eferences: Waalwijk, C., Flavell, R.A., Nucl. Acids Res.5: 3231-3236 (1978). 
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007 
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006 
 M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Mammalian chromosomal DNA digestion with restriction endonucleases in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 29-38, 2006 
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
 
Fragment lengths
 
2293, 2168, 1989, 1570, 1252, 1172, 1049, 851, 771, 654, 649, 621, 615, 599, 510, 488, 459, 437, 427, 426, 417, 383, 378, 377, 370, 363, 358, 347, 339, 337, 335, 334, 306, 299, 287, 286, 285, 282, 270, 267, 265, 259, 250, 243, 240, 238, 237, 236, 235, 232, 227, 223, 222, 220, 219, 213, 206, 203, 202, 200, 194, 193, 192, 191, 189, 185, 184, 182, 181, 179, 175, 173, 171, 168, 161, 159, 154, 153, 152, 151, 149, 145, 143, 141, 138, 134, 130, 129, 124, 123, 121, 120, 118, 117, 116, 114, 111, 108, 107, 105, 103, 102, 101, 99, 95, 94, 91, 90, 89, 88, 87, 86, 84, 83, 82, 81, 78, 76, 75, 74, 72, 71, 70, 69, 68, 67, 65, 63, 62, 61, 60, 59, 58, 57,
LAMBDA DNA
Close
 
M 1 2 3 4 5 M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322

 

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