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Index / Bioassays / EnzyChrom™ α-Amylase Assay Kit, Quantitative determination of α-amylase activity based on colorimetric glucose determination at 585nm / Product Detail : ECAM-100 EnzyChrom™ α-Amylase Assay Kit, Quantitative determination of α-amylase activity based on colorimetric glucose determination at 585nm
#ECAM-100 EnzyChrom™ α-Amylase Assay Kit, Quantitative determination of α-amylase activity based on colorimetric glucose determination at 585nm
Product name : EnzyChrom™ α-Amylase Assay Kit, Quantitative determination of α-amylase activity based on colorimetric glucose determination at 585nm
Catalog number : ECAM-100
Supplier name : Bioassays
Data sheet: Ask more or other datasheet now !
About this Product :EnzyChrom™ α-Amylase Assay Kit, Quantitative determination of α-amylase activity based on colorimetric glucose determination at 585nm antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of EnzyChrom™ α-Amylase Assay Kit, Quantitative determination of α-amylase activity based on colorimetric glucose determination at 585nm.
More Details aboutThe EnzyChrom™ α-Amylase Assay Kit is made for quantitative determination of α-amylase activity. Sensitive and accurate. Linear detection range 0.3 to 50 U/L α-amylase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, incubation for 15 min, followed by the detection reagent and a 20-min incubation and reading the optical density at 585 nm. AMYLASE belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases (EC 184.108.40.206) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure. Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. The EnzyChrom™ a-amylase assay method involves two steps: (1). α-amylase in the sample hydrolyzes starch and the product is rapidly converted to glucose by α-glucosidase and hydrogen peroxide by glucose oxidase; (2). hydrogen peroxide concentration is determined with a colorimetric reagent.#2#http://biotech-map.com/aa_search/table_sample.html?q=%CE%B1-Amylase%20Assay%20Kit&filter=
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WP291: Sex determination
WP1004: Kit Receptor Signaling Pathway
WP109: UDP-Glucose Conversion
WP1121: Kit Receptor Signaling Pathway
WP1341: Kit Receptor Signaling Pathway
WP1455: Serotonin Transporter Activity
WP147: Kit Receptor Signaling Pathway
WP1488: CFTR activity in the plasma membrane
WP1601: Fluoropyrimidine Activity
WP1602: Nicotine Activity on Dopaminergic Neurons
WP1603: Nicotine Activity on Chromaffin Cells
WP1688: Polyketide sugar unit biosynthesis
WP1703: Streptomycin biosynthesis
WP1765: Mechanosensory inputs influence pharyngeal activity via ivermectin sensitivity genes.
WP1899: Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs)
WP1935: Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds
WP2061: Fates of Glucose-6-P
WP2083: Nicotine Activity on Dopaminergic Neurons
WP2248: anthocyanin biosynthesis
WP2344: vitamin B6 (pyridoxine, pyridoxal, pyridoxamine) biosynthesis and salvage pathway
WP259: Nifedipine Activity
WP260: Glucose-1-phosphate metabolism
WP312: Pentose Phosphate Pathway
WP340: Glucose Fermentation
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