GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Bioassays / SuperLight™ Dual Luciferase Reporter Gene Assay Kit, Bioluminescent reagent system allows rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells within 10 m / Product Detail : SLDL-100 SuperLight™ Dual Luciferase Reporter Gene Assay Kit, Bioluminescent reagent system allows rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells within 10 m
Related keywords:

Sep
30th

#SLDL-100 SuperLight™ Dual Luciferase Reporter Gene Assay Kit, Bioluminescent reagent system allows rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells within 10 m

Ask technical file .

  Price : 196   EUR
223   USD
152   GBP
826   Zloty
26337   JPY
1518   NOK
1626   SEK
222   CHF

Product name : SuperLight™ Dual Luciferase Reporter Gene Assay Kit, Bioluminescent reagent system allows rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells within 10 m

Catalog number : SLDL-100

Quantity: 100Tests

Availability: Yes

Supplier name : Bioassays

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

About this Product :

SuperLight™ Dual Luciferase Reporter Gene Assay Kit, Bioluminescent reagent system allows rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells within 10 m antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of SuperLight™ Dual Luciferase Reporter Gene Assay Kit, Bioluminescent reagent system allows rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells within 10 m.
http://mybiofast.com/ver.php?search=anti-&submit=Search

More Details about

Bioluminescent reagent system for rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells.
Method: Lu.
Samples: cells etc.
Species: all.
Procedure: 10 min.
Size: 100 tests.
Detection limit: 2 fg luciferase.

DESCRIPTION
The accuracy of reporter assays can be improved by utilizing a dual
reporter system. One of the reporter genes is correlated with the promoter
of interest and is used to assess the effects of specific experimental
conditions, while the second reporter is used as a control and serves as a
baseline response. The SuperLightTM Dual-Luciferase Reporter Gene
Assay allows for the sequential measurement of the activity of two
different luciferases, firefly (FFL) and Renilla (RL), in a single sample. The
firefly luciferase luminescence is measured first by addition of the FFL
Reagent. Next, the RL Reagent is added to the same well. The RL
Reagent simultaneously quenches the firefly luciferase luminescence and
initiates the Renilla luciferase reaction. The light production of both
reactions can be conveniently measured on a luminometer.
Reaction schemes:
ATP + D-luciferin + O2 oxyluciferin + AMP + PPi + CO2 + light
Firefly Luciferase
Coelenterazine + O2 Coelenteramide + CO2 + light Renilla Luciferase
This bioluminescent dual reporter gene assay is extremely sensitive and
is especially suitable for quantifying dual luciferase expression in
recombinant cells or in cell free transcription/translation reactions. Assays
can be performed in tubes, cuvettes or multi-well plates.
KEY FEATURES
High sensitivity and wide detection range: detection of as little of 2 fg
luciferase.
Compatible with routine laboratory and HTS formats: assays can be
performed in tubes or microplates, and measured with any luminometer.
Can be readily automated on HTS liquid handling systems.
Fast and convenient: three step assay allows detection of dual
luciferase levels within 20 minutes.
APPLICATIONS
Gene Regulation: gene expression level, characterization of promoter
activity, modulation of gene expression by receptors, transcription factors
and small molecules.
Drug Discovery: high-throughput screen for gene modulators.
KIT CONTENTS (100 ASSAYS IN 96-WELL PLATES)
Lysis Buffer: 25 mL FFL Reagent: 25 mg
Assay Buffer: 25 mL RL Reagent: 400 mg
Custom bulk reagent available upon request.
Storage conditions. The kit is shipped at ambient temperature. Store all
Reagents at -20°C. Shelf life: 12 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Bioassays \ SuperLight™_Dual_Luciferase_Reporter_Gene_Assay_Kit,_Bioluminescent_reagent_system_allows_rapid_quantitation_of_firelfy_and_Ranilla_luciferase_reporter_gene_expression_in_transfected_cells_within_10_m \ SLDL_100
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur