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Index / SBI / Exosomes ExoQuick TC 50ml / Product Detail : EXOTC50A-1 Exosomes ExoQuick TC 50ml
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#EXOTC50A-1 Exosomes ExoQuick TC 50ml

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Product name : Exosomes ExoQuick TC 50ml

Catalog number : EXOTC50A-1

Quantity: 50 ml

Availability: Yes

Supplier name : SBI

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The quantity of protein was determined by the Bradford microassay method (Bio-Rad Laboratories), using BSA as a standard. Proteins from each exosome isolate were standardized to the original sample volume and equal volumes were applied per lane of a 12.5% SDS-PAGE gel. Western immunoblotting was performed to analyze the presence of the specific marker protein, placental alkaline phosphatase (PLAP). The SDS-PAGE gel was transferred to a nitrocellulose membrane, the membrane blocked for 1 hour at room temperature with non-fat dried milk, and probed overnight at 4°C with primary antibody. The bound immune complexes were visualized by enhanced chemiluminescence (ECL, Amersham Life Sciences) and quantitated by densitometry (Un-Scan-it Software, Silk Scientific Corp.).

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