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Index / SBI / MicroRNA Tools miRZip-21 anti-miR-21 microRNA construct / Product Detail : MZIP21-PA-1 MicroRNA Tools miRZip-21 anti-miR-21 microRNA construct
#MZIP21-PA-1 MicroRNA Tools miRZip-21 anti-miR-21 microRNA construct
Product name : MicroRNA Tools miRZip-21 anti-miR-21 microRNA construct
Catalog number : MZIP21-PA-1
Quantity: Bacterial Streak
Supplier name : SBI
Data sheet: Ask more or other datasheet now !
About this Product :MicroRNA Tools miRZip-21 anti-miR-21 microRNA construct antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of MicroRNA Tools miRZip-21 anti-miR-21 microRNA construct.
MicroRNA Tools miRZip-21 anti-miR-21 microRNA construct storing antibdies for longer storage periods of the MicroRNA Tools miRZip-21 anti-miR-21 microRNA construct we recommend - 21C or lower and for short periods lees than 1 month we suggest +5C fridge temperatures.
MicroRNA Tools miRZip-21 anti-miR-21 microRNA construct rna research rna is not so stable and very sticky. Rnases are abundand on hans and everywhere. SoreRNA at -75C and use rnase free.
More Details about
Suppression of cell invasion and tumor metastasis by miRZip-21 and increased metastasis using miRZip-145
To investigate the roles of miR-21 (oncogenic microRNA) and miR-145 (tumor suppressor microRNA) in breast cancer, cell invasion assays of MDA-MB-231 breast cancer cells were performed after lentiviral transduction with control vector lentivirus (Control) or miRZip-21 or miRZip-145 lentivirus. Matrigel chamber assays were then used to screen for invasive phenotypes.
After 20 h, invasion cells attached to the lower surface were fixed, followed by staining with 0.05% crystal violet. The number of invaded cells on the membrane was then counted under a microscope. Representative fields of invasive cells on the membrane are shown at the bottom for the Control and miRZip-21 or miRZip-145 treated cells.
The miRZip-21 lentivirus expressing anti-mir-21 small RNAs inhibited metastasis by 80% and the miRZip-145 lentivirus expressing anti-miR-145 RNAs increased metastasis by 60%. Additionally, the miRZip-145 lentivirus inhibited endogenous miR-145 and elevated protein expression levels of the miR-145 target oncogene c-Myc.
• List of miRZip clones
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Related Genes :
[AGO2 EIF2C2] Protein argonaute-2 (Argonaute2) (hAgo2) (EC 3.1.26.n2) (Argonaute RISC catalytic component 2) (Eukaryotic translation initiation factor 2C 2) (eIF-2C 2) (eIF2C 2) (PAZ Piwi domain protein) (PPD) (Protein slicer)
[FMR1] Synaptic functional regulator FMR1 (Fragile X mental retardation protein 1) (FMRP) (Protein FMR-1)
[DICER1 DICER HERNA KIAA0928] Endoribonuclease Dicer (EC 126.96.36.199) (Helicase with RNase motif) (Helicase MOI)
[Fmr1 Fmr-1] Synaptic functional regulator FMR1 (Fragile X mental retardation protein 1 homolog) (FMRP) (Protein FMR-1) (mFmr1p)
[MIR17HG C13orf25 MIRH1 MIRHG1] Putative microRNA 17 host gene protein (Putative microRNA host gene 1 protein)
[Sirt1 Sir2l1] NAD-dependent protein deacetylase sirtuin-1 (EC 3.5.1.-) (Regulatory protein SIR2 homolog 1) (SIR2-like protein 1) (SIR2alpha) (Sir2) (mSIR2a) [Cleaved into: SirtT1 75 kDa fragment (75SirT1)]
[ZC3H12A MCPIP MCPIP1] Endoribonuclease ZC3H12A (EC 3.1.-.-) (Monocyte chemotactic protein-induced protein 1) (MCP-induced protein 1) (MCPIP-1) (Regnase-1) (Reg1) (Zinc finger CCCH domain-containing protein 12A)
[DROSHA RN3 RNASE3L RNASEN] Ribonuclease 3 (EC 188.8.131.52) (Protein Drosha) (Ribonuclease III) (RNase III) (p241)
[Lin28a Lin28 Tex17] Protein lin-28 homolog A (Lin-28A) (Testis-expressed protein 17)
[TARBP2 TRBP] RISC-loading complex subunit TARBP2 (TAR RNA-binding protein 2) (Trans-activation-responsive RNA-binding protein)
[ZFP36 G0S24 NUP475 RNF162A TIS11A TTP] mRNA decay activator protein ZFP36 (G0/G1 switch regulatory protein 24) (Growth factor-inducible nuclear protein NUP475) (Tristetraprolin) (Zinc finger protein 36) (Zfp-36)
[LIN28A CSDD1 LIN28 ZCCHC1] Protein lin-28 homolog A (Lin-28A) (Zinc finger CCHC domain-containing protein 1)
[Ezh2 Enx1h] Histone-lysine N-methyltransferase EZH2 (EC 184.108.40.206) (ENX-1) (Enhancer of zeste homolog 2)
[Ago2 Eif2c2 Kiaa4215] Protein argonaute-2 (Argonaute2) (mAgo2) (EC 3.1.26.n2) (Argonaute RISC catalytic component 2) (Eukaryotic translation initiation factor 2C 2) (eIF-2C 2) (eIF2C 2) (Piwi/argonaute family protein meIF2C2) (Protein slicer)
[Dicer1 Dicer Mdcr] Endoribonuclease Dicer (EC 220.127.116.11) (Double-strand-specific ribonuclease mDCR-1)
[DRB1 HYL1 At1g09700 F21M12.9] Double-stranded RNA-binding protein 1 (Protein HYPONASTIC LEAVES 1) (dsRNA-binding protein 1) (AtDRB1)
[Fmr1 FXR CG6203] Synaptic functional regulator FMR1 (Fragile X mental retardation syndrome-related protein 1) (dFMR1)
[DGCR8 C22orf12 DGCRK6 LP4941] Microprocessor complex subunit DGCR8 (DiGeorge syndrome critical region 8)
[TNRC6A CAGH26 KIAA1460 TNRC6] Trinucleotide repeat-containing gene 6A protein (CAG repeat protein 26) (EMSY interactor protein) (GW182 autoantigen) (Protein GW1) (Glycine-tryptophan protein of 182 kDa)
[PNPT1 PNPASE] Polyribonucleotide nucleotidyltransferase 1, mitochondrial (EC 18.104.22.168) (3'-5' RNA exonuclease OLD35) (PNPase old-35) (Polynucleotide phosphorylase 1) (PNPase 1) (Polynucleotide phosphorylase-like protein)
[AGO1 EIF2C1] Protein argonaute-1 (Argonaute1) (hAgo1) (Argonaute RISC catalytic component 1) (Eukaryotic translation initiation factor 2C 1) (eIF-2C 1) (eIF2C 1) (Putative RNA-binding protein Q99)
[IGF2BP1 CRDBP VICKZ1 ZBP1] Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2 mRNA-binding protein 1) (IMP-1) (IMP1) (Coding region determinant-binding protein) (CRD-BP) (IGF-II mRNA-binding protein 1) (VICKZ family member 1) (Zipcode-binding protein 1) (ZBP-1)
[UBC24 PHO2 At2g33770 T1B8.8] Probable ubiquitin-conjugating enzyme E2 24 (EC 22.214.171.124) (AtPHO2) (E2 ubiquitin-conjugating enzyme 24) (Ubiquitin carrier protein 24) (Ubiquitin-protein ligase 24)
[Zfp36 Nup475 Tis11 Tis11a Ttp] mRNA decay activator protein ZFP36 (Growth factor-inducible nuclear protein NUP475) (TPA-induced sequence 11) (Tristetraprolin) (Zinc finger protein 36) (Zfp-36)
[Fmr1] Synaptic functional regulator FMR1 (Fragile X mental retardation protein 1 homolog) (FMRP) (Protein FMR-1)
[RAN ARA24 OK/SW-cl.81] GTP-binding nuclear protein Ran (Androgen receptor-associated protein 24) (GTPase Ran) (Ras-like protein TC4) (Ras-related nuclear protein)
[mael CG11254] Protein maelstrom
[APOBEC3G MDS019] DNA dC->dU-editing enzyme APOBEC-3G (EC 3.5.4.-) (APOBEC-related cytidine deaminase) (APOBEC-related protein) (ARCD) (APOBEC-related protein 9) (ARP-9) (CEM-15) (CEM15) (Deoxycytidine deaminase) (A3G)
[ADAR ADAR1 DSRAD G1P1 IFI4] Double-stranded RNA-specific adenosine deaminase (DRADA) (EC 126.96.36.199) (136 kDa double-stranded RNA-binding protein) (p136) (Interferon-inducible protein 4) (IFI-4) (K88DSRBP)
[Zcchc11 Kiaa0191 Tut4] Terminal uridylyltransferase 4 (TUTase 4) (EC 188.8.131.52) (Zinc finger CCHC domain-containing protein 11)
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