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Index / Arbor / Glutathione Fluorescent 384-Well Detection Kit / Product Detail : K006-F1D Glutathione Fluorescent 384-Well Detection Kit
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#K006-F1D Glutathione Fluorescent 384-Well Detection Kit

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  Price : 498   EUR
565   USD
386   GBP
2094   Zloty
66703   JPY
3844   NOK
4119   SEK
563   CHF

Product name : Glutathione Fluorescent 384-Well Detection Kit

Catalog number : K006-F1D

Quantity: 2x384 well plate

Availability: Yes

Supplier name : Arbor

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More Details about

FEATURES

  •  
  •  
  • USE - Measure Free or Oxidized Glutathione in 15 Minutes.
  • SAMPLE - Lysates, WB, RBCs, Serum, Plasma, Urine and Tissue
  • SAMPLES/KIT - ~183 (Free and Oxidized) in Duplicate
  • STABILITY - Stable 4˚C Liquid Reagents

DESCRIPTION

The DetectX® Glutathione (GSH) Fluorometric kit utilizes a proprietary non-fluorescent molecule, ThioStar®, that covalently binds to the free thiol group on GSH to yield a highly fluorescent product. After mixing the sample or standard with ThioStar® and incubating at room temperature for 15 minutes. This kit uses 2 384-well plates that allow the measurement of about 375 samples in duplicate for either GSH or GSSG content, or measure about 183 samples in duplicate for both GSH and GSSG. The signal produced is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. Sample GSSG is determined by addition of a reaction mixture converts all the GSSG into free GSH, which then reacts with ThioStar®.

Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios.

384-Well Glutathione Fluorescent Standard Curve

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