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#SLLU-500 SuperLight™ Luciferase Reporter Gene Assay Kit, Bright bioluminescent reagent system allows rapid quantitation of luciferase reporter gene expression in transfected cells within 2 min. Kit size 500 t

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Product name : SuperLight™ Luciferase Reporter Gene Assay Kit, Bright bioluminescent reagent system allows rapid quantitation of luciferase reporter gene expression in transfected cells within 2 min. Kit size 500 t

Catalog number : SLLU-500

Quantity: 500tests

Availability: Yes

Supplier name : Bioassays

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Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens.
Method: Lu.
Samples: cells etc.
Species: all.
Procedure: 2 min.
Size: 500 tests.
Detection limit: 2 fg luciferase.

DESCRIPTION
The SuperLightTM Luciferase Reporter Gene Assay is based on the
quantitation of luciferase expression in mammalian, yeast or E. coil cells,
using luciferin and ATP as substrates. The reaction results in light
production which can be conveniently measured on a luminometer.
Reaction scheme:
Luciferase
ATP + D-luciferin + O2 oxyluciferin + AMP + PPi + CO2 + light
This bioluminescent reporter gene assay is extremely sensitive and is
especially suitable for quantifying luciferase expression in recombinant
cells. This ultra-sensitive, homogeneous cell-based assay only requires
adding a single reagent to the cells and measuring the light intensity after a
short incubation step (2 minutes). Assays can be performed in tubes,
cuvettes or multi-well plates. All kit components are compatible with culture
media and with all liquid handling systems. With an extended luminescence
emission kinetics (half-life 40 min), the SuperLightTM luciferase assays are
especially suitable for high-throughput screening in 96-well, 384-well and
1536-well plates. In addition, the reagent provided in the kits has been
formulated for maximum sensitivity, reproducibility and long shelf-life.
Applications for this kit include gene regulation studies and high-throughput
screening of gene modulators.
KEY FEATURES
High sensitivity and wide detection range: detection of as little of 2 fg
luciferase and as few as 4 cells. Plus, the emitted light is linear over seven
orders of magnitude.
Compatible with routine laboratory and HTS formats: assays can be
performed in tubes or microplates, on LJL Analyst, Berthold Luminometer,
Top-Count, MicroBeta counters, chemiluminescent image plate readers
(CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling
systems.
Fast and convenient: homogeneous “mix-and-measure” assay allows
detection of luciferase levels within 10 minutes. The optimally combined
reagent system allows a single addition step, and simultaneous cell lysis
and detection.
Robust and amenable to HTS: Z’ factors of 0.6 to 0.8 are observed in 96-
well and 384-well plates. Can be readily automated on HTS liquid handling
systems.
APPLICATIONS
Gene Regulation: gene expression level, characterization of promoter
activity, modulation of gene expression by receptors, transcription factors
and small molecules.
Drug Discovery: high-throughput screen for gene modulators.
KIT CONTENTS
Storage conditions. The kit is shipped at ambient temperature. Store the
Reagent in the provided amber tube at -20°C and the Assay Buffer at 2-8°C.
Shelf life: 12 months after receipt.
This protocol can be downloaded online at www.bioassaysys.com.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.

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