GENTAUR Molecular Products.
Monoclonal Antibody, ELISA Kit, Polyclonal Antibody, Recombinant/Purified Protein.
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Index / Sceti K.K. / E.coli RecA Protein / Product Detail : 01-001 E.coli RecA Protein
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Sep
23th
#01-001 E.coli RecA Protein
| Price : | 261 | EUR |
| 296 | USD | |
| 202 | GBP | |
| 1096 | Zloty | |
| 34926 | JPY | |
| 2013 | NOK | |
| 2156 | SEK | |
| 295 | CHF |
Product name : E.coli RecA Protein
Catalog number : 01-001
Quantity: 200ug
Availability: Yes
Supplier name : Sceti K.K.
Data sheet: Ask more or other datasheet now !
More Details about
Description
E. coli RecA protein is a very important enzyme for homologous recombination and recombinational
repair. Expression is induced by SOS response caused by DNA damage. The RecA protein has
multiple functions, such as single stranded DNA dependent ATPase activity, DNA annealing activity,
formation of D-loops and Holliday structures during homologous recombination, and co-protease
activities that promote self-cleavage of the LexA repressor, lambda phage repressor and UmuD
protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It
carries out a central role in homologous recombination. Its is homologous to the eukaryotic Rad51
protein and Dmcl protein (1).
The product is over-expressed as a recombinant protein and highly purified by several steps of
chromatography. A single band is observed by SDS-PAGE at 38 kD (Fig. 1).
Usage
- Studies of the homologous recombination mechanism and SOS response.
- To facilitate hybridization during library screening using a DNA probe (2).
- Facilitate observation of nucleofilament formation of DNA by electron microscopy.
Specifications
Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.9 mg/ml as measured by BCA method
Form: 50% glycerol, 20 mM Tris-HCl (pH 8.0), 1 mM EDTA, 150
mM KCl, ７mM mercaptoethanol
Storage: -20 C
References
1. Waker GC, Cold Spring Harb. Symp. Quant Biol. 65:1-10
(2000)
2. Taidi-Laskowski B, Nucleic Acids Res. 16:8157-69 (1988)
Fig. 1: Polyacrylamide gel electrophoresis of RecA protein.
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