GENTAUR Molecular Products.
Monoclonal Antibody, ELISA Kit, Polyclonal Antibody, Recombinant/Purified Protein.GENTAUR Belgium BVBA BE0473327336
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Index / Sceti K.K. / E.coli RecA Protein / Product Detail : 01-001 E.coli RecA Protein
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Apr
21th
#01-001 E.coli RecA Protein
Price : | 261 | EUR |
296 | USD | |
202 | GBP | |
1096 | Zloty | |
34926 | JPY | |
2013 | NOK | |
2156 | SEK | |
295 | CHF |
Product name : E.coli RecA Protein
Catalog number : 01-001
Quantity: 200ug
Availability: Yes
Supplier name : Sceti K.K.
Data sheet: Ask more or other datasheet now !
More Details about
Description
E. coli RecA protein is a very important enzyme for homologous recombination and recombinational
repair. Expression is induced by SOS response caused by DNA damage. The RecA protein has
multiple functions, such as single stranded DNA dependent ATPase activity, DNA annealing activity,
formation of D-loops and Holliday structures during homologous recombination, and co-protease
activities that promote self-cleavage of the LexA repressor, lambda phage repressor and UmuD
protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It
carries out a central role in homologous recombination. Its is homologous to the eukaryotic Rad51
protein and Dmcl protein (1).
The product is over-expressed as a recombinant protein and highly purified by several steps of
chromatography. A single band is observed by SDS-PAGE at 38 kD (Fig. 1).
Usage
- Studies of the homologous recombination mechanism and SOS response.
- To facilitate hybridization during library screening using a DNA probe (2).
- Facilitate observation of nucleofilament formation of DNA by electron microscopy.
Specifications
Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.9 mg/ml as measured by BCA method
Form: 50% glycerol, 20 mM Tris-HCl (pH 8.0), 1 mM EDTA, 150
mM KCl, 7mM mercaptoethanol
Storage: -20 C
References
1. Waker GC, Cold Spring Harb. Symp. Quant Biol. 65:1-10
(2000)
2. Taidi-Laskowski B, Nucleic Acids Res. 16:8157-69 (1988)
Fig. 1: Polyacrylamide gel electrophoresis of RecA protein.
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Pathways :
WP2272: Pathogenic Escherichia coli infection
WP1032: NLR proteins
WP1049: G Protein Signaling Pathways
WP1071: Cytoplasmic Ribosomal Proteins
WP1165: G Protein Signaling Pathways
WP1187: Cytoplasmic Ribosomal Proteins
WP1239: Cytoplasmic Ribosomal Proteins
WP1256: NLR proteins
WP1294: NLR proteins
WP1360: NLR proteins
WP1371: G Protein Signaling Pathways
WP1438: Influenza A virus infection
WP1493: Carbon assimilation C4 pathway
WP1502: Mitochondrial biogenesis
WP1531: Vitamin D synthesis
WP1566: Citrate cycle (TCA cycle)
WP1613: 1,4-Dichlorobenzene degradation
WP1616: ABC transporters
WP1624: Bacterial secretion system
WP1625: Base excision repair
WP163: Cytoplasmic Ribosomal Proteins
WP1644: DNA replication
WP1650: Fluorobenzoate degradation
WP1654: gamma-Hexachlorocyclohexane degradation
WP1657: Glycerolipid metabolism
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[tir espE E2348C_3941] Translocated intimin receptor Tir (Secreted effector protein Tir)
[Apc2 APC APC2 apc2 D-APC2 d-APC2 dAPC2 dApc2 dapc2 Dm APC2 Dmel\CG6193 E-APC e-apc E-APC dAPC2 CG6193 Dmel_CG6193] Adenomatous polyposis coli 2 (Adenomatous polyposis coli 2, isoform A) (Adenomatous polyposis coli 2, isoform C) (Epithelial adenomatous polyposis coli)
[espC E2348C_2915] Serine protease EspC (EC 3.4.21.-) [Cleaved into: Secreted autotransporter protein EspC (EPEC-secreted protein C); Autotransporter protein EspC translocator]
[rseA mclA yfiJ b2572 JW2556] Anti-sigma-E factor RseA (Regulator of SigE) (Sigma-E anti-sigma factor RseA) (Sigma-E factor negative regulatory protein)
[gapA b1779 JW1768] Glyceraldehyde-3-phosphate dehydrogenase A (GAPDH-A) (EC 1.2.1.12) (NAD-dependent glyceraldehyde-3-phosphate dehydrogenase)
[APC2 APCL] Adenomatous polyposis coli protein 2 (Adenomatous polyposis coli protein-like) (APC-like)
[rne ams hmp1 b1084 JW1071] Ribonuclease E (RNase E) (EC 3.1.26.12)
[rpoE sigE b2573 JW2557] ECF RNA polymerase sigma-E factor (RNA polymerase sigma-E factor) (Sigma-24)
[sucB b0727 JW0716] Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61) (2-oxoglutarate dehydrogenase complex component E2) (OGDC-E2) (Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex)
[entE b0594 JW0586] Enterobactin synthase component E (EC 6.3.2.14) (2,3-dihydroxybenzoate-AMP ligase) (DHB-AMP ligase) (2,3-dihydroxybenzoate-AMP synthase) (EC 2.7.7.58) (Dihydroxybenzoic acid-activating enzyme) (Enterochelin synthase E) (S-dihydroxybenzoyltransferase) (EC 2.5.1.-)
[aceE b0114 JW0110] Pyruvate dehydrogenase E1 component (PDH E1 component) (EC 1.2.4.1)
[acpP b1094 JW1080] Acyl carrier protein (ACP) (Cytosolic-activating factor) (CAF) (Fatty acid synthase acyl carrier protein)
[cysG E2348C_3618] Siroheme synthase [Includes: Uroporphyrinogen-III C-methyltransferase (Urogen III methylase) (EC 2.1.1.107) (SUMT) (Uroporphyrinogen III methylase) (UROM); Precorrin-2 dehydrogenase (EC 1.3.1.76); Sirohydrochlorin ferrochelatase (EC 4.99.1.4)]
[glmU E2348C_4040] Bifunctional protein GlmU [Includes: UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23) (N-acetylglucosamine-1-phosphate uridyltransferase); Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)]
[yjeF nnrD nnrE E2348C_4490] Multifunctional fusion protein [Includes: ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC 4.2.1.136) (ADP-dependent NAD(P)HX dehydratase); NAD(P)H-hydrate epimerase (EC 5.1.99.6) (NAD(P)HX epimerase)]
[fadB E2348C_4158] Fatty acid oxidation complex subunit alpha [Includes: Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC 4.2.1.17) (EC 5.1.2.3) (EC 5.3.3.8); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)]
[rseP ecfE yaeL b0176 JW0171] Regulator of sigma-E protease RseP (EC 3.4.24.-) (S2P endopeptidase) (Site-2 protease RseP) (S2P protease RseP) (Site-2-type intramembrane protease)
[ppnP E2348C_0327] Pyrimidine/purine nucleoside phosphorylase (EC 2.4.2.2) (Adenosine phosphorylase) (Cytidine phosphorylase) (Guanosine phosphorylase) (EC 2.4.2.15) (Inosine phosphorylase) (Thymidine phosphorylase) (EC 2.4.2.4) (Uridine phosphorylase) (EC 2.4.2.3) (Xanthosine phosphorylase)
[ispH lytB yaaE b0029 JW0027] 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HMBPP reductase) (EC 1.17.7.4) (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase)
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[lpdA lpd b0116 JW0112] Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (Dihydrolipoamide dehydrogenase) (E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes) (Glycine cleavage system L protein)
[arnA E2348C_2399] Bifunctional polymyxin resistance protein ArnA [Includes: UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13) (ArnAFT) (UDP-L-Ara4N formyltransferase); UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylating (EC 1.1.1.305) (ArnADH) (UDP-GlcUA decarboxylase) (UDP-glucuronic acid dehydrogenase)]
[pspE b1308 JW1301] Thiosulfate sulfurtransferase PspE (TST) (EC 2.8.1.1) (Phage shock protein E)
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