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Index / Sceti K.K. / Substance P / Product Detail : 4014 Substance P
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Dec
10th

#4014 Substance P

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  Price : 2235   EUR
2536   USD
1733   GBP
9387   Zloty
299019   JPY
17234   NOK
18466   SEK
2527   CHF

Product name : Substance P

Catalog number : 4014

Quantity: 100mg

Availability: Yes

Supplier name : Sceti K.K.

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More Details about

For the cultivation of non-fastodious microorganisms in water, feces and from clinical samples.
 
FORMULA IN g/l
Gelatin Peptone 5.00
Bacteriological Agar 15.00
Beef Extract 3.00
Final pH 6.8 ± 0.2 at 25ºC
 
PREPARATION
Suspend 23 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. The prepared medium should be stored at 8-15°C. The color is amber, slightly opalescent. The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes, discard the medium.
 
USES
NUTRIENT AGAR is a general purpose medium, not selective but suitable for the cultivation of a wide variety nonfastidious microorganisms. It can be used as a colony count medium in sanitation, medica and industrial bacteriology.
There are many uses for Nutrient Agar in the bacteriological analysis of drinking water, wastewater, milk and other foods. The American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing.
It is also used in the multiplication of microorganisms to produce vaccines and antigens in general, in the tests of sensitivity and resistance, and as a base to prepare an enriched medium by adding ascitic fluid, etc. It is used to grow microorganisms and for subsequent biochemical tests.
The Gelatin peptone and Beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth.
Bacteriological agar is the solidifying agent.
ISO 6579, ISO 19250 and ISO 10273 recommend this medium to obtain presuntive Salmonella and Yersinia isolated colonies respectively. A typical or suspicious colony from each selective medium must be seeded, and then the other four if the first one turns out to be negative. In the case of epidemiological studies, it is recommended to identify at least five colonies. Should there be less than five typical or suspicious colonies on a plate, all the typical or suspicious colonies will be used for confirmation.
Inoculate medium with the test sample and incubate at 35 ± 2°C for 18 – 48 hours. Good growth will appear as translucent colonies.
According ISO 19250 incubate at 36 ± 2°C for 24 ± 3 hours.
 
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 35 ± 2°C and observed after 18 - 48 hours.
 
Microorganisms Growth
Staphylococcus aureus ATCC 25923 Good
Escherichia coli ATCC 25922 Good
Salmonella typhimurium ATCC 14028 Good
Streptococcus pyogenes ATCC 12344 Good
Streptococcus pneumoniae ATCC 6301 Good
*Yersinia enterocolitica ATCC 23715 Good
*According ISO 10273 incubate at 30ºC during 24 hours.
According ISO 19250 incubate at 36 ± 2°C for 24 ± 3 hours.
 
BIBLIOGRAPHY
Greenberg and Cooper Can. Med. Assn. J. 83:143. 1960. Wetmore and Gochenour J. Bact. 72:79, 1956
Norma UNE-EN-ISO 6579. Microbiology of food and animal feeding stuffs -- Horizontal method for the detection of Salmonella spp.
ISO 10273-2003-06 Microbiology of Food and animal feeding stuffs- Horizontal method for the detection of presumptive pathogenic 
Yersinia enterocolitica.
ISO 19250 water quality-detection of Salmonella spp
 
STORAGE
Once opened keep powdered medium closed to avoid hydration.

 

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