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#3433-005-01 Cultrex BME, no phenol red, reduced growth factor
Product name : Cultrex BME, no phenol red, reduced growth factor
Catalog number : 3433-005-01
Quantity: 5 ml
Supplier name : Trevigen
Data sheet: Ask more or other datasheet now !
More Details about
Cultrex® BME, reduced growth factor without phenol red
Catalog #: 3433-005-01
Cultrex® Basement Membrane Extract
Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at 37°C to form a reconstituted basement membrane. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycan. Trevgen's BME typically ranges in protein concentrations from 14 to 16 mg/ml. Trevgen's BME typically ranges in protein concentrations from 14 to 16 µg/ml. BME is commonly used to maintain stem and precursor cells in an undifferentiated state during cell culture and BME hydrogels function to induce differentiation of epithelial, endothelial and smooth muscle cells in vitro. BME has also been employed in cell attachment, neurite outgrowth, angiogenesis, and in vitro cell invasion assays. BME is available with and without Phenol Red. Cultrex® BME is also available in PathClear® and HC20+™ varieties.
Endotoxin (LPS) Concentration: ≤ 8 EU/ml
Source: Murine Engelbreth-Holm-Swarm (EHS) tumor
Storage Buffer: Dulbecco’s Modified Eagle’s medium containing 10 μg/ml gentamycin sulfate +/- phenol red.
Storage/Stability: Product is stable for a minimum of 3 months from date of shipment when stored at –20 °C in a manual defrost freezer. For optimal stability, store at –80 °C in aliquots. Keep Frozen; repeated freeze-thaws will destroy product integrity.
Trevgen's BME typically ranges in protein concentrations from 14 to 16 mg/ml.
Tube Formation Assay: Basement Membrane Extract promotes formation of capillary-like structures by human (HUVEC, HMVEC) or mouse (SVEC4-10) endothelial cells.
3-D Culture: Basement Membrane Extract promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) and human prostate (PC-3) into acinar structures.
STANDARD STERILITY TESTING:
No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP XXIV Chapter 71 sterility test.
No mycoplasma contamination detected by PCR.
Endotoxin concentrations ≤ 8 EU/ml by LAL assay.
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WP1046: Signaling of Hepatocyte Growth Factor Receptor
WP1162: Signaling of Hepatocyte Growth Factor Receptor
WP1206: Signaling of Hepatocyte Growth Factor Receptor
WP1235: Signaling of Hepatocyte Growth Factor Receptor
WP1789: Binding of RNA by Insulin-like Growth Factor-2 mRNA Binding Proteins (IGF2BPs/IMPs/VICKZs)
WP1899: Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs)
WP193: Signaling of Hepatocyte Growth Factor Receptor
WP2256: Integrated Pancreatic Cancer Pathway
WP2377: Integrated Pancreatic Cancer Pathway
WP313: Signaling of Hepatocyte Growth Factor Receptor
WP444: Signaling of Hepatocyte Growth Factor Receptor
WP810: Signaling of Hepatocyte Growth Factor Receptor
WP927: Signaling of Hepatocyte Growth Factor Receptor
WP94: Signaling of Hepatocyte Growth Factor Receptor
WP1006: metapathway biotransformation
WP1065: Endochondral Ossification
WP107: Translation Factors
WP1084: Translation Factors
WP110: Translation Factors
WP1124: metapathway biotransformation
WP1181: Endochondral Ossification
WP1212: metapathway biotransformation
WP1220: Translation Factors
WP1251: metapathway biotransformation
WP1270: Endochondral Ossification
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