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Index / Trevigen / 3_D Culture Matrix / Product Detail : 3445-001-01 3_D Culture Matrix
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Dec
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#3445-001-01 3_D Culture Matrix

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  Price : 126   EUR
143   USD
97   GBP
530   Zloty
16890   JPY
973   NOK
1043   SEK
142   CHF

Product name : 3_D Culture Matrix

Catalog number : 3445-001-01

Quantity: 1 ml

Availability: Yes

Supplier name : Trevigen

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More Details about

3-D Culture Matrix
 


3-D Culture Matrix™ reduced growth factor (RGF) BME

3-D Culture Matrix™ reduced growth factor (RGF) BME provides the foundation for cells to grow in three dimensions, allowing for the formation of acinar and other hollow structures in vitro. Trevigen's 3-D Culture Matrix™ products enable investigators to employ an innovative in vitro approach to modeling cell responses in in vivo-like environments, which cannot be observed in standard 2-D cultures1-3.

Specifications:

CONCENTRATION :

12–18 mg/ml

SOURCE:

Murine Engelbreth-Holm-Swarm (EHS) tumor

STORAGE BUFFER:

Dulbecco's Modified Eagle's medium containing 10 μg/ml gentamicin sulfate and no phenol red.

STORAGE/STABILITY:

Product is stable for a minimum of 3 months from date of shipment when stored at -20°C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles.

Material Qualification:

FUNCTIONAL ASSAY:

  • Acinar structure formation: 3-D Culture Matrix™ BME promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
  • Tube Formation Assay: BME promotes formation of capillary-like structures by human (HUVEC, HMVEC) or mouse (SVEC4-10) endothelial cells.

STERILITY TESTING:

  •  No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP XXIV Chapter 71 sterility test.
  •  No mycoplasma contamination detected by PCR.
  •  Endotoxin concentrations ≤ 8 EU/ml by LAL assay.

REFERENCES:

1. Benton, G., and J. George. 2008. Using 3-D culture for high-throughput analysis of cytotoxicity and proliferation. Am Biotechnol Lab 26:24-7.
2. Benton G., E. Crooke, and J. George. 2009. Laminin-1 induces E-cadherin expression in 3-dimensional cultured breast cancer cells by inhibiting DNA methyltransferase 1 and reversing promoter methylation status. FASEB J. 23:3884-95.
3. Benton G, J. George, H.K. Kleinman, I.P. Arnaoutova. 2009. Advancing science and technology via 3D culture on basement membrane matrix. J Cell Physiol. 221:18-25.

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