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Index / Trevigen / Cultrex In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit / Product Detail : 3471-096-K Cultrex In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit
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#3471-096-K Cultrex In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit

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  Price : 421   EUR
478   USD
327   GBP
1770   Zloty
56397   JPY
3250   NOK
3482   SEK
476   CHF

Product name : Cultrex In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit

Catalog number : 3471-096-K

Quantity: 96 tests

Availability: Yes

Supplier name : Trevigen

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During angiogenesis, which is the formation of neovasculature associated with wound healing and a host of disease states including tumor growth, endothelial cells invade through the basement membrane to form sprouting vessels, which in turn invade hypoxic tissue. Trevigen's Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit was created in an effort to accelerate the screening process for compounds that influence vascular endothelial cell digestion through a basement membrane extract (BME) layer. This kit offers a flexible, standardized, 96 well high-throughput format for quantitating endothelial invasion, in vitro, in response to chemoattractants and/or inhibiting compounds. This assay employs a simplified Boyden chamber-like design with an 8 micron polyethylene terephthalate (PET) membrane. Sulforaphane [1-isothiocyanato-(4R)-methylsulfinyl)-butane], a naturally occurring cancer chemopreventive agent, is provided as a control for inhibition of in vitro endothelial cell invasion through Cultrex® BME. Detection of cell invasion is quantified using Calcein-AM. Calcein AM is internalized by the cells, and intracellular esterases cleave the acetomethylester (AM) moiety. Free Calcein fluoresces brightly, and this fluorescence may be used to quantitate the number of cells that have invaded or migrated using a standard curve.

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