GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Trevigen / Thermostable TDG Enzyme & Buffer / Product Detail : 4070-500-EB Thermostable TDG Enzyme & Buffer
Related keywords:

Dec
6th

#4070-500-EB Thermostable TDG Enzyme & Buffer

Ask technical file .

  Price : 392   EUR
445   USD
304   GBP
1649   Zloty
52532   JPY
3027   NOK
3244   SEK
444   CHF

Product name : Thermostable TDG Enzyme & Buffer

Catalog number : 4070-500-EB

Quantity: 500 Units

Availability: Yes

Supplier name : Trevigen

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

Description: TDG is a thermostable thymine DNA glycosylase from Methanobacterium thermoautotrophicum. The optimal temperature for the enzyme is 65°C. The enzyme lacks significant AP lyase or endonuclease activity. TDG works effectively in heteroduplex analysis to detect C to T transitions.
Source: Thermostable TDG is purified from E. coli containing a recombinant plasmid harboring the Methanobacterium thermoautotrophicum TDG gene.
Unit Definition: One Unit is the amount of enzyme required to cleave 1 pmole of an oligonucleotide duplex containing a T/G mismatch in 1 hour at 65°C. Only the strand containing the T is cleaved.
Substrate Specificity: TDG enzyme recognizes T/G mismatches in duplex DNA and cleaves the strand with the T. The opposite strand is not cleaved. The enzyme also recognizes G/G mismatches if at least one nearest neighbor is an A or T and nicks one strand or the other. The enzyme exhibits poor AP lyase activity.
Assay Conditions & Analysis: 4 pmoles of T/G mismatch oligonucleotide set with the T oligo end-labeled, 1X REC™ Buffer 4 (10 mM HEPES-KOH (pH 7.4), 100 mM KCl, and 10mM EDTA), and serial dilutions of enzyme in a 20 μl reaction volume are incubated for 1 hour at 65°C. To complete cleavage of abasic site, fresh 1N NaOH is added to final concentration of 166 mM then heated for 15 minutes at 95°C. For analysis, 24 μl of 2X Loading Buffer (20 mM EDTA, 95% formamide, and 0.13% bromophenol blue)are added, and the samples heated at 95°C for 10 min then fast cooled to 4°C. The cleavage products are resolved by 20% denaturing polyacrylamide gel electrophoresis, and percent cleavage quantified.
Storage Buffer: 25 mM HEPES (pH 7.4), 100 mM NaCl, 1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol.
Storage Conditions: Store at -20°C in a manual defrost freezer. For long term storage, freeze in working aliquots at -80°C. Avoid repeated freeze-thawing. Enzyme may be diluted in 1X REC Buffer 4 for immediate use. TDG protein in storage buffer can survive for up to 24 hours at 37°C with less than 10% loss in activity.

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Trevigen \ Thermostable_TDG_Enzyme_&_Buffer \ 4070_500_EB
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur