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#4110-01K-EB Human AP Endonuclease & Buffer
Product name : Human AP Endonuclease & Buffer
Catalog number : 4110-01K-EB
Quantity: 1000 Units
Supplier name : Trevigen
Data sheet: Ask more or other datasheet now !
More Details about
Description: Human APE (also referred to as Ref-1) is a 37 kDa multifunctional enzyme. It is involved in both DNA repair and in facilitating the redox state for a number of DNA binding proteins.
Source: Purified from E. coli containing a recombinant plasmid harboring the hAPE gene.
Unit Definition: One unit cleaves 1 pmole of a labeled oligonucleotide probe containing an AP site within an oligonucleotide duplex at the rate of 1 pmol/hour at 37°C.
Specificity: Human APE is responsible for the repair of apurinic/apyrimidinic (AP) sites in the DNA base excision repair (BER) pathway. It catalyzes the cleavage of the phosphodiester bond immediately 5’ to an AP site. APE also functions as a redox factor facilitating the DNA-binding capability of JUN, FOS, AP-1, NF-kB, and other transcription factors. The APE-related DNA repair activity has been demonstrated to be inactivated by phosphorylation, suggesting that this might be the mechanism by which the enzyme switches between the two functions.
Assay Conditions: 1X REC Buffer 7 (10 mM HEPES-KOH (pH 7.4), 100 mM KCl, 10 mM MgCl2), 4 pmole of a labeled AP oligonucleotide containing an AP site, annealed to a complement oligonucleotide, and serial dilutions of enzyme in a 20 μl reaction volume are incubated for 1 hour at 37°C. The cleavage products are resolved by 20% denaturing polyacrylamide gel electrophoresis, and the percent cleavage quantified.
Storage Buffer: 10 mM HEPES-KOH (pH 7.4), 100 mM KCl, 0.1 mg/ml BSA, 50% (v/v) glycerol.
Storage Conditions: Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots. Avoid repeated freeze-thawing.
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WP2107: pathways in human cancer
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[ELANE ELA2] Neutrophil elastase (EC 22.214.171.124) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase)
[RACK1 GNB2L1 HLC7 PIG21] Receptor of activated protein C kinase 1 (Cell proliferation-inducing gene 21 protein) (Guanine nucleotide-binding protein subunit beta-2-like 1) (Guanine nucleotide-binding protein subunit beta-like protein 12.3) (Human lung cancer oncogene 7 protein) (HLC-7) (Receptor for activated C kinase) (Small ribosomal subunit protein RACK1) [Cleaved into: Receptor of activated protein C kinase 1, N-terminally processed (Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed)]
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[FBN1 FBN] Fibrillin-1 [Cleaved into: Asprosin]
[CTNNB1 CTNNB OK/SW-cl.35 PRO2286] Catenin beta-1 (Beta-catenin)
[CCNDBP1 DIP1 GCIP HHM] Cyclin-D1-binding protein 1 (Grap2 and cyclin-D-interacting protein) (Human homolog of Maid)
[F8 F8C] Coagulation factor VIII (Antihemophilic factor) (AHF) (Procoagulant component) [Cleaved into: Factor VIIIa heavy chain, 200 kDa isoform; Factor VIIIa heavy chain, 92 kDa isoform; Factor VIII B chain; Factor VIIIa light chain]
[APP A4 AD1] Amyloid beta A4 protein (ABPP) (APPI) (APP) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Beta-amyloid precursor protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99; Beta-amyloid protein 42 (Beta-APP42); Beta-amyloid protein 40 (Beta-APP40); C83; P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID(59)) (Gamma-CTF(59)); Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID(57)) (Gamma-CTF(57)); Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)); C31]
[BRCA1 RNF53] Breast cancer type 1 susceptibility protein (EC 188.8.131.52) (RING finger protein 53) (RING-type E3 ubiquitin transferase BRCA1)
[AKAP13 BRX HT31 LBC] A-kinase anchor protein 13 (AKAP-13) (AKAP-Lbc) (Breast cancer nuclear receptor-binding auxiliary protein) (Guanine nucleotide exchange factor Lbc) (Human thyroid-anchoring protein 31) (Lymphoid blast crisis oncogene) (LBC oncogene) (Non-oncogenic Rho GTPase-specific GTP exchange factor) (Protein kinase A-anchoring protein 13) (PRKA13) (p47)
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[HBB] Hemoglobin subunit beta (Beta-globin) (Hemoglobin beta chain) [Cleaved into: LVV-hemorphin-7; Spinorphin]
[SIRT1 SIR2L1] NAD-dependent protein deacetylase sirtuin-1 (hSIRT1) (EC 3.5.1.-) (Regulatory protein SIR2 homolog 1) (SIR2-like protein 1) (hSIR2) [Cleaved into: SirtT1 75 kDa fragment (75SirT1)]
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[LRRK2 PARK8] Leucine-rich repeat serine/threonine-protein kinase 2 (EC 220.127.116.11) (Dardarin)
[TGFB1 TGFB] Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)]
[AKT1 PKB RAC] RAC-alpha serine/threonine-protein kinase (EC 18.104.22.168) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha)
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