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Index / Trevigen / Human AP Endonuclease & Buffer / Product Detail : 4110-05K-EB Human AP Endonuclease & Buffer
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#4110-05K-EB Human AP Endonuclease & Buffer

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  Price : 465   EUR
528   USD
361   GBP
1954   Zloty
62265   JPY
3588   NOK
3845   SEK
526   CHF

Product name : Human AP Endonuclease & Buffer

Catalog number : 4110-05K-EB

Quantity: 5000 Units

Availability: Yes

Supplier name : Trevigen

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More Details about

Description: Human APE (also referred to as Ref-1) is a 37 kDa multifunctional enzyme. It is involved in both DNA repair and in facilitating the redox state for a number of DNA binding proteins.
Source: Purified from E. coli containing a recombinant plasmid harboring the hAPE gene.
Unit Definition: One unit cleaves 1 pmole of a labeled oligonucleotide probe containing an AP site within an oligonucleotide duplex at the rate of 1 pmol/hour at 37°C.
Specificity: Human APE is responsible for the repair of apurinic/apyrimidinic (AP) sites in the DNA base excision repair (BER) pathway. It catalyzes the cleavage of the phosphodiester bond immediately 5’ to an AP site. APE also functions as a redox factor facilitating the DNA-binding capability of JUN, FOS, AP-1, NF-kB, and other transcription factors. The APE-related DNA repair activity has been demonstrated to be inactivated by phosphorylation, suggesting that this might be the mechanism by which the enzyme switches between the two functions.
Assay Conditions: 1X REC Buffer 7 (10 mM HEPES-KOH (pH 7.4), 100 mM KCl, 10 mM MgCl2), 4 pmole of a labeled AP oligonucleotide containing an AP site, annealed to a complement oligonucleotide, and serial dilutions of enzyme in a 20 μl reaction volume are incubated for 1 hour at 37°C. The cleavage products are resolved by 20% denaturing polyacrylamide gel electrophoresis, and the percent cleavage quantified.
Storage Buffer: 10 mM HEPES-KOH (pH 7.4), 100 mM KCl, 0.1 mg/ml BSA, 50% (v/v) glycerol.
Storage Conditions: Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots. Avoid repeated freeze-thawing.

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