GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Trevigen / Human Fen_1 Enzyme and Buffer / Product Detail : 4120-100-EB Human Fen_1 Enzyme and Buffer
Related keywords:

Jul
30th

#4120-100-EB Human Fen_1 Enzyme and Buffer

Ask technical file .

  Price : 425   EUR
483   USD
330   GBP
1788   Zloty
56969   JPY
3283   NOK
3518   SEK
481   CHF

Product name : Human Fen_1 Enzyme and Buffer

Catalog number : 4120-100-EB

Quantity: 100 Units

Availability: Yes

Supplier name : Trevigen

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

Description: FEN-1 is a 50 kDa endonuclease/exonuclease that functions in mammalian replication and repair. The enzyme shows similarity to the yeast Rad2 and Rad13 genes. FEN-1 was identified as a necessary component of Okazaki fragment processing and functions on a number of branched DNA structures. It has been shown that FEN-1 specifically associates with PCNA and this binding stimulates FEN-1 up to 50 fold.
Source: Purified from E. coli containing a recombinant plasmid encoding the human FEN-1 protein.
Unit Definition: One Unit cleaves 10 pmole of a 32P-labeled oligonucleotide flap complex in one hour at 30°C.
Specificity: FEN-1 catalyzes the nucleotide excision of DNA branch structures formed during replication of the lagging strand of DNA. The branch structures are formed due to the frequent mismatches that occur at the 5'-end of Okazaki fragments. FEN-1 is also involved in the endonucleolytic cleavage of branch structures that are formed during DNA double strand break end-joining. FEN-1 cleaves DNA flap structures one nucleotide distal to the elbow in the single-stranded region or one nucleotide proximal to the elbow in the double-stranded region, creating two possible cleavage products (see figure overleaf).
FEN-1 also has a 5'-3' exonuclease activity specific for recessed 5' ends. This exonuclease activity may be involved in the removal of initiator RNA of mammalian Okazaki fragments.
Assay Conditions: The flap substrate is prepared by hybridizing 100 pmole Flap Oligonucleotide labeled with 32P, 200 pmole Bridge Oligonucleotide, and 200 pmole Adjacent Oligonucleotide in 20 mM Tris (pH 7.4),15 mM NaCl in a 100 μl volume at 99°C for 10 minutes and then cooling slowly to 4°C. 1X REC™ Reaction Buffer 12, 1X BSA Additive (0.1 mg/ml BSA, 5% glycerol), 8 μl of the above 32P flap substrate, and serial dilutions of enzyme in a reaction volume of 20 μl are incubated for 1 hour at 30°C. For analysis, 5 μl of 5X REC™Loading Buffer (Cat# 4018-250: 20 mM EDTA, 20% Ficoll, and 0.2% bromophenol blue) are added, and cleavage products are resolved by 20% denaturing polyacrylamide gel electrophoresis. The bands are cut out and radioactivity counted to quantify the cleavage products.
Storage Conditions: Store at -20°C in a manual defrost freezer.
Storage Buffer: 50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 1 mM DTT, 0.1 mg/ml BSA, and 50% (v/v) glycerol.

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Trevigen \ Human_Fen_1_Enzyme_and_Buffer \ 4120_100_EB
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur