GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Trevigen / Human Ku70_Ku80 / Product Detail : 4135-250-01 Human Ku70_Ku80
Related keywords:

Aug
28th

#4135-250-01 Human Ku70_Ku80

Ask technical file .

  Price : 434   EUR
493   USD
336   GBP
1824   Zloty
58114   JPY
3349   NOK
3589   SEK
491   CHF

Product name : Human Ku70_Ku80

Catalog number : 4135-250-01

Quantity: 250 units

Availability: Yes

Supplier name : Trevigen

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

Description: Human Ku 70/80 complex consists of 69.7 kDa and 82.6 kDa subunits, both of which act independently as ATP-dependent DNA helicases. The complex binds at double stranded DNA breaks and recruits DNA-PK, RAD50-Mre11-Xrs2, and XRCC4/DNA ligase IV to the site to facilitate nonhomologous end joining (NHEJ). Ku 70/80 complex also participates in NHEJ during V(D)J recombination of immunoglobulin genes, which is necessary for generating the large antibody repertoire. The complex also restores activity to an immuno-depleted cell free DNA repair assay.
Source: Recombinant Ku heterodimer.
Unit Definition: One Unit is the amount of Ku 70/80 complex required to shift 100 fmoles of a labeled oligonucleotide duplex in 30 minutes at 25°C in an electrophoretic mobility shift assay.
Specificity: Ku 70/80 complex binds to ends of double stranded DNA breaks
Assay Conditions:
Duplex oligonucleotides: 1 pmole of an end labeled 24-mer oligonucleotide was incubated with 2 pmoles of a complementary oligonucleotide in 25 mM Tris-Cl (pH 7.8), 0.5 mM EDTA, 5 mM MgCl2, 10 % glycerol, and 0.1% bromophenol blue in a reaction volume of 10 ml for 10 minutes at 65°C, and slow cooled to 22°C.
Gel shift: Serial dilutions of the Ku 70/80 complex were incubated with the oligonucleotide duplex at 25°C for 30 minutes. Products were resolved on a 5% non-denaturing polyacrylamide gel.
Storage Buffer: 50 mM Tris-Cl (pH 7.9), 0.3 M KCl, 1 mM EDTA, 0.02 % Tween 20, 15% glycerol, 1 mM DTT, and 10 mg/ml PMSF.
Storage Conditions: Store in working aliquots at -80˚C. Avoid repeated freeze thaws.

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Trevigen \ Human_Ku70_Ku80 \ 4135_250_01
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur