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#4250-050-K CometAssay Kit
Product name : CometAssay Kit
Catalog number : 4250-050-K
Quantity: 50 Samples
Supplier name : Trevigen
Data sheet: Ask more or other datasheet now !
More Details about
- Applicable for the analysis of either single strand or double strand DNA breaks
- Study cellular responses to DNA damage
- Screen for inhibitors of DNA repair
- Test chemicals for toxicity
- Screen for environmental mutagens
- Probe for specific types of DNA damage using DNA repair glycosylases
Trevigen's CometAssay®, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA "comet" tail shape and migration pattern allows for assessment of DNA damage. The Neutral CometAssay® is typically used to detect double-stranded breaks, whereas the Alkaline CometAssay® is more sensitive, and is used to detect smaller amounts of damage includingsingle and double-stranded breaks.
ASSAY DESIGN FOR ALKALINE COMET:
Step 1: Cells mixed with low melting agarose are immobilized on specially treated slides and lysed.
- Membranes and histones removed from the DNA of individually isolated cells.
Step 2: Samples are treated with alkali to unwind and denature the DNA.
- For detection of single and double-stranded breaks.
Step3: Following alkaline electrophoresis slides are stained and visualized by fluorescent microscopy.
- Increase in DNA damage results in smaller fragments migrating faster in an electric field.
- Quantitative measurements of DNA damage
Catalog # Description
4250-050-01 Lysis Solution
4250-050-02 Comet LMAgarose
4250-050-03 CometSlide™ (2 well)
4250-050-04 200 mM EDTA
4250-050-05 SYBR® Green
Trevigen will no longer be including SYBR® Green (4250-050-05) in the CometAssay® Kits or offer it for individual purchase effective September 4th, 2012.
You may want to check other SYBR Green that Gentaur has in its offer:
|20010||SYBR Green I Gel Staining Solution, 10000x||1 mL|
|20010-2||SYBR Green I Gel Staining Solution, 10000x||2 x 1 mL|
|30010-3||SYBR Green I Gel Staining Solution, 10000x||3 x 1.5 mL|
|30010-5||SYBR Green I Gel Staining Solution, 10000x||5 x 1.5 mL|
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52062 Aachen Deutschland
Support Karolina Elandt
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IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
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Related Genes :
 miR-183 Inhibits UV-Induced DNA Damage Repair in Human Trabecular Meshwork Cells by Targeting of KIAA0101.
 Evaluating the effects of galbanic acid on hydrogen peroxide-induced oxidative DNA damage in human lymphocytes.
 In Barrett's esophagus patients and Barrett's cell lines, ursodeoxycholic acid increases antioxidant expression and prevents DNA damage by bile acids.
 Robust classification of DNA damage patterns in single cell gel electrophoresis.
 Inhibition of autophagy as a strategy to augment radiosensitization by the dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitor NVP-BEZ235.
 In benign Barrett's epithelial cells, acid exposure generates reactive oxygen species that cause DNA double-strand breaks.
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