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Index / Trevigen / CometAssay 20 Well ES Starter Kit plus Power Supply for North America / Product Detail : 4252-040-ESK-PS1 CometAssay 20 Well ES Starter Kit plus Power Supply for North America
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#4252-040-ESK-PS1 CometAssay 20 Well ES Starter Kit plus Power Supply for North America

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  Price : 2592   EUR
2942   USD
2011   GBP
10888   Zloty
346828   JPY
19989   NOK
21419   SEK
2931   CHF

Product name : CometAssay 20 Well ES Starter Kit plus Power Supply for North America

Catalog number : 4252-040-ESK-PS1

Quantity: -

Availability: Yes

Supplier name : Trevigen

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More Details about

CometAssay® 20 Well ES Unit w/ Starter Kit
Catalog #: 4252-040-ESK

COMETASSAY® ELECTROPHORESIS SYSTEM STARTER KIT

Trevigen makes available a complete comet assay system which includes CometAssay® kits,CometSlides®, Comet Assay® control cells and a specialized electrophoresis unit for alkaline and neutral comet assays.

CometAssay® Kit, 40 Samples (2X20 well slides)
Catalog #: 4252-040-K

APPLICATIONS:

  • Applicable for the analysis of either single strand or double strand DNA breaks                                                                                
  • Study cellular responses to DNA damage
  • Screen for inhibitors of DNA repair
  • Test chemicals for toxicity
  • Screen for environmental mutagens
  • Probe for specific types of DNA damage using DNA repair glycosylases

Trevigen's CometAssay®, or single cell gel electrophoresis assay, provides a simple andeffective method for evaluating DNA damage in cells.  The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied.  Evaluation of the DNA "comet" tail shape and migration pattern allows for assessment of DNA damage.  The Neutral CometAssay® is typically used to detect double-stranded breaks, whereas the AlkalineCometAssay® is more sensitive, and is used to detect smaller amounts of damage includingsingle and double-stranded breaks.

ASSAY DESIGN FOR ALKALINE COMET:

Step 1: Cells mixed with low melting agarose are immobilized on specially treated slides and lysed.

  • Membrane and histones removed from the DNA of individually isolated cells.

Step 2: Samples are treated with alkali to unwind and denature the DNA.

  • For detection of single and double-stranded breaks.

Step3: Following alkaline electrophoresis slides are stained and visualized by fluorescent microscopy.

  • Increase in DNA damage results in smaller fragments migrating faster in an electric field.
  • Quantitative measurements of DNA damage

COMPONENTS:

Catalog # Description
4250-050-01 Lysis Solution
4250-050-02 Comet LMAgarose
4250-050-03 CometSlide™ (2 well)
4250-050-04 200 mM EDTA
4250-050-05 SYBR® Green

Trevigen will no longer be including SYBR® Green (4250-050-05)  in the CometAssay® Kits or offer it for individual purchase effective September 4th, 2012.

 

You may want to check other SYBR Green that Gentaur has in its offer:

20010 SYBR Green I Gel Staining Solution, 10000x 1 mL  
20010-2 SYBR Green I Gel Staining Solution, 10000x 2 x 1 mL  
30010-3 SYBR Green I Gel Staining Solution, 10000x 3 x 1.5 mL  
30010-5 SYBR Green I Gel Staining Solution, 10000x 5 x 1.5 mL

 

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