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#4253-096-K CometAssay Kit 96 wells
Product name : CometAssay Kit 96 wells
Catalog number : 4253-096-K
Quantity: 96 samples
Supplier name : Trevigen
Data sheet: Ask more or other datasheet now !
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CometAssay® 96 Well ES Unit w/ Starter Kit
Catalog #: 4253-096-ESK
COMETASSAY® ELECTROPHORESIS SYSTEM STARTER KIT
Trevigen makes available a complete assay system which includes CometAssay® kits,CometSlides®, Comet Assay® control cells and a specialized electrophoresis unit for alkaline and neutral comet assays.
STARTER KIT (ESK) COMPONENTS
Catalog # Component
CometAssay® Kit 96 Samples (1X96 well slide)
Catalog #: 4253-096-K
Trevigen's CometAssay®, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA "comet" tail shape and migration pattern allows for assessment of DNA damage. The Neutral CometAssay® is typically used to detect double-stranded breaks, whereas the AlkalineCometAssay® is more sensitive, and is used to detect smaller amounts of damage includingsingle and double-stranded breaks.
ASSAY DESIGN FOR ALKALINE COMET:
Step 1: Cells mixed with low melting agarose are immobilized on specially treated slides and lysed.
Step 2: Samples are treated with alkali to unwind and denature the DNA.
Step 3: Following alkaline electrophoresis slides are stained and visualized by fluorescent microscopy.
Catalog # Description
Trevigen will no longer be including SYBR® Green (4250-050-05) in the CometAssay® Kits or offer it for individual purchase effective September 4th, 2012.
You may want to check other SYBR Green that Gentaur has in its offer:
|20010||SYBR Green I Gel Staining Solution, 10000x||1 mL|
|20010-2||SYBR Green I Gel Staining Solution, 10000x||2 x 1 mL|
|30010-3||SYBR Green I Gel Staining Solution, 10000x||3 x 1.5 mL|
|30010-5||SYBR Green I Gel Staining Solution, 10000x||5 x 1.5 mL|
CometAssay® Electrophoresis System
Trevigen's CometAssay® Electrophoresis System enables investigators to consistently optimize alkaline and neutral comet assays for highly reproducible results, and to standardize electrophoresis methods between individual users and laboratories. Comet assay results can be variable depending on temperature, distance between electrodes, and buffer height. Trevigen has solved these problems by developing a specialized electrophoresis unit.
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