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Index / Trevigen / Anti_PAR Monoclonal Antibody w_Control / Product Detail : 4335-MC-100-AC Anti_PAR Monoclonal Antibody w_Control
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Jul
26th

#4335-MC-100-AC Anti_PAR Monoclonal Antibody w_Control

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  Price : 438   EUR
497   USD
340   GBP
1842   Zloty
58687   JPY
3382   NOK
3624   SEK
496   CHF

Product name : Anti_PAR Monoclonal Antibody w_Control

Catalog number : 4335-MC-100-AC

Quantity: 100 ul

Availability: Yes

Supplier name : Trevigen

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About this Product :

Anti_PAR Monoclonal Antibody w_Control http://biotech-map.com/aa_search/table_sample.html?q=monoclonal&filter=
Description
Anti_PAR Monoclonal Antibody w_Control is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.

More Details about

Description: Trevigen’s monoclonal antibody (cat# 4335-MC-100) is specific for poly(ADPribose) (PAR) polymer. It can be used to detect ribosylated proteins by ELISA, Western blot, immunocytochemistry in situ, and for immunopurification. It is supplied with a positive control for ELISA and Western blot consisting of a poly-ADP-ribosylated PARP-1 protein (PARP-PAR).
Storage Conditions: Store the anti-PAR monoclonal at -20°C (manual defrost freezer). Store the PARP-treated protein control at -80°C.
Physical State: This antibody is provided as purified immunoglobulin from mouse ascites in 1X PBS containing 50% glycerol at 1 mg/ml. PARP-PAR is provided at 75 μM in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA.
Immunogen: Purified ADP-ribose polymers between 2 and 50 units long
Ig Class: IgG3
Specificity: The antibody is specific for PAR polymers 2 to 50 units long, but does not recognize structurally related RNA, DNA, ADP-ribose monomers, NAD, or other nucleic acid monomers.
Applications: ELISA, Western analysis, immunoprecipitation, and immunopurification. For Western blots, an antibody dilution of 1:1000 is recommended.

Procedure for Immunoblotting using Peroxidase Detection:
Blotting buffer: 12 mM Tris base, 96 mM Glycine, and 15% MeOH.
Blocking solution: 5% (w/v) nonfat dry milk in TBS-0.1% Tween.
Antibody solution: 5% (w/v) nonfat dry milk, in TBS-0.1% Tween.


Transfer the electrophoresed proteins to an Immobilon-FL or PVDF membrane and incubate the membrane for 1 hour at room temperature in blocking solution.
Incubate the membrane overnight at 4°C in antibody solution containing a 1:1000 dilution of Trevigen’s anti-PAR monoclonal antibody. Empirical determination of the optimal monoclonal antibody concentration will be required for optimal results.


Wash the membrane at room temperature for 5 minutes with 4 changes of TBS-0.1% Tween. Changing the membrane containers often reduces background.
Incubate the membrane at room temperature for 1 hour in antibody solution containing antirabbit conjugated to horseradish peroxidase, or IR800-conjugated secondary antibody (Licor).
Empirical determination of secondary antibody concentration will be required for optimal results.
Wash the membrane for 15 minutes with 4 changes of TBS-0.1% Tween.
Develop peroxidase reaction using chemiluminescence, or as described in the figure 2
legend.

 

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