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Index / Trevigen / 8_oxo_dG Elisa Kit / Product Detail : 4370-096-K 8_oxo_dG Elisa Kit
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Sep
26th

#4370-096-K 8_oxo_dG Elisa Kit

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  Price : 1056   EUR
1198   USD
819   GBP
4435   Zloty
141279   JPY
8142   NOK
8725   SEK
1194   CHF

Product name : 8_oxo_dG Elisa Kit

Catalog number : 4370-096-K

Quantity: 96 samples

Availability: Yes

Supplier name : Trevigen

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About this Product :

8_oxo_dG Elisa Kit
http://mybiofast.com/ver.php?search=ELISA+kit&submit=Search
ELISA kits expiry date
ELISA kits are stored between 3 and 9 C. The expiry date is mostly affected by the standard. We can supply standards separately if you suspect the storage was at too high temperature or if your lot nr has passed the expiry date and if you still want to work with the same 8_oxo_dG Elisa Kit lot number. For more technical support about $$ contact us at info@gentaur.com

More Details about

 

HT 8-oxo-dG ELISA
Catalog #: 4370-096-K

HT 8-oxo-dG ELISA Kit

8-hydroxy-2’-deoxyguanosine (8-oxo-dG) is a modified nucleoside, which is the most commonly studied and detected by-product of DNA damage that is excreted following DNA repair. The presence of 8-oxo-dG and its analogs, 8-hydroxyguanosine and 8-hydroxyguanine in the urine, is associated with many degenerative disease states in which reactive oxygen species (ROS) contribute to pathogenesis. 8-oxo-dG, a biomarker of oxidative stress, has been employed as an ROS indicator in many studies on a wide variety of diseases.

Trevigen’s 8-oxo-dG ELISA (enzyme-linked immunosorbent assay) is a fast and sensitive, competitive immunoassay for the detection and quantitation of 8-hydroxy-2'-deoxyguanosine (8-oxo-dG) in saliva, urine and serum samples. This kit features a 96 strip well plate pre-bound with 8-oxo-dG, an 8-oxo-dG monoclonal antibody, an enzyme-labeled secondary antibody, detection substrates and buffer to provide a complete, robust assay flexible for your experimental design.

FEATURES:

  • Colorimetric format
  • High throughput 96 test size
  • Standard curve has a range of 0.94 - 60 ng/ml
  • Sensitive to 0.59 ng/ml

APPLICATIONS :

For the detection and quantitation of 8-hydroxy-2'-deoxyguanosine (8-oxo-dG) in urine, serum and saliva samples.

STORAGE:

All reagents are stable as supplied at 4°C, except the 8-oxo-dG standard, which should be stored at -20°C. For optimum storage, the 8-oxo-dG standard should be aliquoted into smaller portions and stored at -20°C to avoid repeated freeze/ thaw cycles. Unused wells of the 8-oxo-dG Immunoassay Plate should be resealed with desiccant in the foil pouch provided and stored at 4°C until the kit expiration date.

REFERENCES:

1. Evans, M. D., Dizdaroglu, M., and Cooke, M. S. (2004) Mutat. Res. 567: 1-61.
2. Sancar, A., Lindsey-Boltz, L. A., Unsal-Kacmaz, K., and Linn, S. (2004) Annu. Rev. Biochem. 73: 39-85.
3. Chiou, C. C., Chang, P. Y., Chan, E. C., Wu, T. L., Tsao, K. C., and Wu, J. T. (2003) Clin. Chim. Acta. 334: 87-94.
4. Trzeciak, A. R., Nyaga, S. G., Jaruga, P., Lohani, A., Dizdaroglu, M., and Evans, M. K. (2004) Carcinogenesis 25: 1359-1370.
5. Brown, R. K., McBurney, A., Lunec, J., and Kelly, F. J. (1995) Free Radic. Biol. Med. 18: 801-806.
6. Tsuboi, H., Kouda, K., Takeuchi, H., Takigawa, M., Masamoto, Y., Takeuchi, M., and Ochi, H. (1998) Br. J. Dermatol. 138: 1033-1035.
7. Rall, L. C., Roubenoff, R., Meydani, S. N., Han, S. N., and Meydani, M. (2000) J. Nutr. Biochem. 11: 581-584.
8. Lezza, A. M., Mecocci, P., Cormio, A., Beal, M. F., Cherubini, A., Cantatore, P., Senin, U., and Gadaleta, M. N. (1999) FASEB J. 13: 1083-1088.
9. Alam, Z. I., Jenner, A., Daniel, S. E., Lees, A. J., Cairns, N., Marsden, C. D., Jenner, P., and Halliwell, B. (1997) J. Neurochem. 69: 1196-1203.

 

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