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Index / Trevigen / PARP in vivo Pharmacodynamic Assay 2nd Generation / Product Detail : 4520-096-K PARP in vivo Pharmacodynamic Assay 2nd Generation
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Sep
25th

#4520-096-K PARP in vivo Pharmacodynamic Assay 2nd Generation

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  Price : 1289   EUR
1463   USD
1000   GBP
5415   Zloty
172483   JPY
9941   NOK
10652   SEK
1458   CHF

Product name : PARP in vivo Pharmacodynamic Assay 2nd Generation

Catalog number : 4520-096-K

Quantity: 96 Samples

Availability: Yes

Supplier name : Trevigen

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More Details about

PARP in vivo Pharmacodynamic Assay 2nd Generation(PDAII)
Catalog #: 4520-096-K


FEATURES:

  • Pre-coated 96 well capture antibody plates
  • High signal to noise ratio
  • Detection sensitivity of 2 pg/ml of PAR
  • Broad linear dynamic range to 1,000 pg/ml
  • Reduced inter-assay variability
  • Validated assay that measures drug action on PARP in both in vivo and in vitro settings
  • 96 test size

PARP catalyzes the NAD+-dependent addition of poly (ADP-ribose) (PAR) onto itself andadjacent nuclear proteins. This enzyme is a therapeutic target for BRCA1 and BRCA2 associated breast cancers. To address the need to monitor PARP activity among different individuals and within cells, Trevigen's improved and validated HT PARP in vivo Pharmacodynamic Assay II measures net PAR levels in tissue or cellular extracts and has been used to document differences in PAR levels among tumor lysates, organs and xenografts. The HT PARP in vivo Pharmacodynamic Assay II employs a 96 well plate, pre-coated with Trevigen's monoclonal PAR antibody as the capture agent, and anti-PAR polyclonal rabbit antibody as the detecting agent.

APPLICATIONS:

  • Quantitation of PAR in peripheral blood mononuclear cells, tissue culture cells, and tumorlysates from different tissues, organs and xenografts.
  • Monitoring the efficacy of PARP inhibitors on PAR formation in vivo.
  • Verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/anticancer drug combination therapy.
  • Facilitating development of PARP and PARG targeted therapeutics.

KIT COMPONENTS:

  • PAR Standard and Sample Buffer PAR Polyclonal Detecting Ab and Diluent
  • Goat anti-Rabbit IgG-HRP Detection Reagents
  • Cell Lysis Reagent and 20% SDS DNase I and Mg Cation
  • Jurkat Cell Lysate Standard Controls
  • (Low, Medium, and High)
  • Pre-coated 96-stripwell plate and sealers

REFERENCES:

1. Virag, L., Szabo, C. 2002. The therapeutic potential of Poly(ADP-Ribose) Polymerase inhibitors. Pharmacological Reviews 54:375-429.

2. Curtin NJ. 2005. PARP inhibitors for cancer therapy. Expert Rev Mol Med 7:1-20.

3. Kinders JK, Hollingshead M, Khin S, Rubinstein L, Tomaszewski JE, Doroshow JH, Parchment RE, and the National Cancer Institute Phase 0 Clinical Trials Team. 2008. Preclinical Modeling of a Phase 0 Clinical Trial: Qualification of a Pharmacodynamic Assay of Poly (ADP-Ribose) Polymerase in Tumor Biopsies of Mouse Xenografts. Clin Cancer Res 14:6877-85.

4. Bryant HE, Schultz N, Thomas HD, Parker KM, Flower D, Lopez E, Kyle S, Meuth M, Curtin NJ, Helleday T. 2005. Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase. Nature 434:913-7.

5. Farmer H, McCabe N, Lord CJ, Tutt AN, Johnson DA, Richardson TB, Santarosa M, Dillon KJ, Hickson I, Knights C, Martin NM, Jackson SP, Smith GC, Ashworth A. 2005. Targeting the DNA repair defect in BRCA mutant cells as a therapeutic strategy. Nature 434:917-21.

6. Fong PC, Boss DS, Yap TA, Tutt A, Wu P, Mergui-Roelvink M, Mortimer P, Swaisland H, Lau A, O'Connor MJ, Ashworth A, Carmichael J, Kaye SB, Schellens JH, de Bono JS. 2009. Inhibition of poly(ADP-ribose) polymerase in tumors from BRCA mutation carriers. N Engl J Med 361:123-34.

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