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Index / Trevigen / HT Colorimetric PARP Apoptosis Assay Kit / Product Detail : 4684-096-K HT Colorimetric PARP Apoptosis Assay Kit
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Dec
5th

#4684-096-K HT Colorimetric PARP Apoptosis Assay Kit

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  Price : 589   EUR
669   USD
457   GBP
2476   Zloty
78870   JPY
4545   NOK
4870   SEK
666   CHF

Product name : HT Colorimetric PARP Apoptosis Assay Kit

Catalog number : 4684-096-K

Quantity: 96 Tests

Availability: Yes

Supplier name : Trevigen

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More Details about

4684-096-K

HT Colorimetric PARP Apoptosis Assay Kit

FEATURES:

  • Colorimetric readout
  • 96 well format
  • Highly sensitive – detects 0.1 mU PARP ~500 cells
  • Dynamic range between 0.1 to 10 mU PARP
  • Requires 10-100 ng extract for detection
  • Assay Time ~3 hrs

During apoptosis, PARP-1 which catalyzes the NAD-dependent addition of poly (ADP-ribose) (PAR) onto various cytoplasmic and nuclear proteins, is cleaved from about 116 kDa to 85 kDa . Moreover, this enzyme is a therapeutic target for BRCA1 and BRCA2 associated breast cancers. The HT PARP/Apoptosis Assay is ideal for measuring the activity of PARP in cell extracts before and during apoptosis. This ELISA semi-quantitatively detects PAR deposited onto immobilized histone proteins in a 96-well format. An anti-PAR monoclonal antibody, goat anti-mouse IgG-HRP conjugate, and HRP substrate are used to generate a signal. Thus, signals correlate with PARP activity. Etoposide is a topoisomerase II inhibitor that stabilizes this enzyme after it cleaves DNA. It is included as a control apoptosis inducer.

APPLICATIONS:

  • Measure activity in cells, primary or tumor cells
  • Measure activity before and after apoptosis
  • Measure effect of PARP inhibitors using cell extracts

REFERENCES:

  1. Lawen A. 2003. Apoptosis—an introduction. BioEssays 25:888-896.
  2. Okada H, Mak TW. 2004. Pathways of apoptotic and non-apoptotic death in tumor cells. Nat Rev Cancer 4:592-603.
  3. Miller MS, Zobre C, Lewis M. 1993. In vitro neuroprotective activity of inhibitors of poly-ADP ribose polymerase. Soc Neurosci Abstr 19.1656
  4. Piper AA, Verma A, Zhang J, Snyder SH. 1999. Poly(ADP-ribose) polymerase, nitric oxide and cell death. Trends in Pharmacological Sciences 20:171-181.
  5. Thiemermann C, Bowes J, Myint FP, and Vane JR. 1997. Inhibition of the activity of poly(ADP-ribose) synthase reduces ischemia-reperfusion injury in the heart and skeletal muscle. Proc Natl Acad Sci USA 94:679-683.
  6. Virag L, Szabo C. 2002. The therapeutic potential of Poly(ADP-Ribose) Polymerase inhibitors.  Pharmacological Reviews 54:375-429.
  7. Baldwin EL, Osherhoff N. 2005. Etoposide, Topoisomerase II and Cancer. Curr. Med. Chem. Anti-Cancer Agents 5:363-372.
  8. Bryant HE, Schultz N, Thomas HD, Parker KM, Flower D, Lopez E, Kyle S, Meuth M, Curtin NJ, Helleday T. 2005. Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase. Nature 434:913-7.
  9. Farmer H, McCabe N, Lord CJ, Tutt AN, Johnson DA, Richardson TB, Santarosa M, Dillon KJ, Hickson I, Knights C, Martin NM, Jackson SP, Smith GC, Ashworth A. 2005. Targeting the DNA repair defect in BRCA mutant cells as a therapeutic strategy. Nature 434:917-21.
  10. Fong PC, Boss DS, Yap TA, Tutt A, Wu P, Mergui-Roelvink M, Mortimer P, Swaisland H, Lau A, O'Connor MJ, Ashworth A, Carmichael J, Kaye SB, Schellens JH, de Bono JS. 2009. Inhibition of poly(ADP-ribose) polymerase in tumors from BRCA mutation carriers. N Engl J Med 361:123-34.

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