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Index / Trevigen / Mesenchymal Stem Cell Adipogenic Differentiation Kit / Product Detail : 5010-024-K Mesenchymal Stem Cell Adipogenic Differentiation Kit
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#5010-024-K Mesenchymal Stem Cell Adipogenic Differentiation Kit

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  Price : 476   EUR
540   USD
369   GBP
1999   Zloty
63697   JPY
3671   NOK
3933   SEK
538   CHF

Product name : Mesenchymal Stem Cell Adipogenic Differentiation Kit

Catalog number : 5010-024-K

Quantity: 24 wells

Availability: Yes

Supplier name : Trevigen

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Mesenchymal Stem Cell Adipogenic Differentaition Kit
Catalog #: 5010-024-K

Cultrex® Mesenchymal Stem Cell Adipogenic Differentiation Kit

Adipogenesis is the process by which fat is formed.(1) Fat formation plays a role in obesity, cardiovascular disease, and metabolic disorders.(1) It also has a role in the maintenance of the bone marrow cavity. Adipocytes are derived from mesenchymal stem cells (MSC), which are a self-renewing population of multipotent cells present in bone marrow and many other adult tissues that can differentiate into multiple lineage-specific cells that form bone, fat, cartilage, muscle and tendon.(2-5) Differentiation of primary cells, specifically MSC, into adipocytes can be a useful tool for understanding the mechanisms involved in adipogenesis leading eventually to interventions designed to prevent obesity.

Trevigen’s adipogenic differentiation kit follows traditional methods of adipogenic differentiation,(6) by growing MSC in medium supplemented with insulin, isobutyl methyl xanthine (IBMX), indomethacin and dexamethasone. The kit will induce adipogenic differentiation of MSC within 14-17 days. Adipogenic differentiation is detected by staining with Oil Red O. Oil Red O will stain lipid containing vacuoles in mesenchymal stem cells that have undergone adipogenic differentiation.

FEATURES:

Optimized reagents for the differentiation of Mesenchymal Stem Cells into Adipocytes. Sufficient reagents to differentiate one 24 well plate and stain two 24 well plates. The amount of differentiation can be quantified.

APPLICATIONS :

Tool for understanding mechanisms involved in adipogenesis eventually leading to interventions designed to prevent obesity.

REFERENCES:

1. Kiess W, Petzold S, Topfer M, Garten A, Bluher, Kapellen T, Korner A, Kratzsch J 2008 Adipocytes and Adipose Tissue. Best Practice and Research Clinical Endocrinology and Metabolism 22(1):135-153.
2. Phinney DG AND Prockop DJ 2007. Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Models of Tissue Repair-Current Views. Stem Cells 25:2896-2902
3. Kolf CM, Cho E, Tuan RS. 2007. Biology of Adult Mesenchymal Stem Cells: Regulation of Niche, Self-Renewal and Differentiation. Arthritis Research and Therapy 9(1): 204-214
4. Javazon EH, Colter DC, Schwarz EJ, Prockop DJ. 2001. Rat Marrow Stromal Cells are More Sensative to Plating Density and Expand More Rapidly from Single-Cell-Derived Colonies than Human Marrow Stromal Cells. Stem Cells 19:219-225
5. Li Yi, McIntosh K, Chen J, Zhang C, Gao, Q, Borneman J, Ragniski K, Mitchell J, Shen L, Zhang J, Lu D, Chopp M. Allogeneic bone marrow stromal cells promote glial-axonal remodeling without immunologic sensitization after stroke in rats. Experimental Neurology 198(2):313-25.
6. Reger RL, Tucker AH, Wolfe MR. 2008. Differentiation and Characterization of Human MSCs. In Prockop DJ, Phinney DG, Bunnell BA (eds) Methods in Molecular Biology Mesenchymal Stem Cells: Methods and Protocols vol 449. Humana Press, Totawa, NJ, USA pp 93-107

 

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