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Index / Trevigen / HT Glutathione Peroxidase Assay Kit / Product Detail : 7512-100-K HT Glutathione Peroxidase Assay Kit
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#7512-100-K HT Glutathione Peroxidase Assay Kit

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  Price : 541   EUR
614   USD
419   GBP
2273   Zloty
72428   JPY
4174   NOK
4473   SEK
612   CHF

Product name : HT Glutathione Peroxidase Assay Kit

Catalog number : 7512-100-K

Quantity: 480 tests

Availability: Yes

Supplier name : Trevigen

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More Details about

HT Glutathione Peroxidase Assay Kit
 


HT Glutathione Peroxidase Assay Kit

FEATURES:

  • Suitable for a 96 well or cuvette format
  • Suitable for plasma, erythrocyte lysates, tissue homogenates, and cell lysates

APPLICATIONS :

Calculating glutathione peroxidase activity

Glutathione peroxidase (GP) is found in mammalian cells and helps to prevent lipid peroxidation of cell membranes by consuming free peroxide in the cell. Thus, Glutathione Peroxidase plays a critical role in protecting the cell from free radical damage. Tetrameric Glutathione Peroxidase catalyzes the reduction of H2O2 to water, and organic peroxides to the corresponding stable alcohols, by using glutathione as a source of reducing equivalents. It requires selenium as a cofactor and contains a selenocysteine amino acid residue in the active site of each monomer that participates in the actual mechanism of the enzyme. Glutathione Reductase (GR), provided with each kit, then reduces the oxidized glutathione to complete the cycle.  The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the Glutathione Peroxidase activity in the sample. Trevigen’s Glutathione Peroxidase Assay Kit can be used to measure glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

REFERENCES:

1. Ozdemir G, Ozden M, Maral H, Kuskay S, Cetinalp P, Tarkun I. Malondialdehyde, glutathione, glutathione peroxidase and homocysteine levels in type 2 diabetic patients with and without microalbuminuria. Ann Clin Biochem. 2005, 42:99-104.
2. Prasad NR, Menon VP, Vasudev V, Pugalendi KV. Radioprotective effect of sesamol on gamma-radiation induced DNA damage, lipid peroxidation and antioxidants levels in cultured human lymphocytes. Toxicology. 2005, 209:225-35.
3. Sindhu RK, Ehdaie A, Farmand F, Dhaliwal KK, Nguyen T, Zhan CD, Roberts CK, Vaziri ND. Expression of catalase and glutathione peroxidase in renal insufficiency. Biochim Biophys Acta. 2005, 1743:86-92.
4. Mukhopadhyay P, Rajesh M, Bátkai S, Kashiwaya Y, Haskó G, Liaudet L, Szabó C, Pacher P. Role of superoxide, nitric oxide, and peroxynitrite in doxorubicin-induced cell death in vivo and in vitro. Am J Physiol Heart Circ Physiol. 2009 296:H1466-8
5. Chu, F.F., R.S. Esworthy, J.H. Doroshow. 2004. Role of Se-dependent glutathione peroxidases in gastrointestinal inflammation and cancer. Free Radic. Biol. Med. 36(12):1481-1495.


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