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Index / Trevigen / HT Glutathione Reductase Assay Kit / Product Detail : 7513-500-K HT Glutathione Reductase Assay Kit
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Aug
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#7513-500-K HT Glutathione Reductase Assay Kit

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  Price : 594   EUR
675   USD
461   GBP
2498   Zloty
79585   JPY
4587   NOK
4915   SEK
672   CHF

Product name : HT Glutathione Reductase Assay Kit

Catalog number : 7513-500-K

Quantity: 480 tests

Availability: Yes

Supplier name : Trevigen

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HT Glutathione Reductase Assay Kit
 


HT Glutathione Reductase Assay Kit

FEATURES:

  • Suitable for cell lysates, erythrocyte lysates, and tissue homogenate
  • Sufficient reagents for 500 assays
  • 96 well format for high throughput screening

APPLICATIONS :

Calculating glutathione reductase activity

This high throughput glutathione reductase assay kit is designed to measure cellular levels of Glutathione Reductase, an enzyme which catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH) (Figure 1). GSH is an important intracellular antioxidant that reacts with oxygen free radicals and organic peroxides and acts as a substrate for other detoxification enzymes such as glutathione peroxidase and glutathione S-transferase. The activity of
glutathione reductase is an important measure of the antioxidant status of the cell. Glutathione reductase requires NADPH for its activity, resulting in the reduction of GSSG to GSH and the corresponding oxidation of NADPH to NADP+.

Trevigen's Glutathione Reductase Assay Kit is a spectrophotometric assay in which the oxidation of NADPH to NADP+ is monitored by the decrease in absorbance at 340 nm. This rate of decrease in absorbance at 340 nm is directly proportional to the glutathione reductase activity in the sample because the enzyme is present at rate limiting concentrations. This kit provides the user with all the reagents and plates to easily and rapidly assay for glutathione reductase in cell and tissue extracts.

REFERENCES:

1. Berg JM, Tymoczko JL, Styer L. 2002. Biochemistry, 5th Edition, WH Freeman and Company, New York.
2. Tietze, F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal. Biochem., 27, 502-522 (1969).
3. Smith IK, Vierheller TL, Thorne CA. Assay of glutathione reductase in crude tissue homogenates using 5,5'-dithiobis(2-nitrobenzoic acid). Anal. Biochem., 175, 408-413 (1988).
4. Dolphin, D, Poulson, R, Avramovic, O. (Eds.), Glutathione: Chemical, Biochemical, and Metabolic Aspects, Volumes A and B, Wiley and Sons (1989).
5. Dringen R, Gutterer JM. Glutathione reductase from bovine brain. Methods Enzymol. 348, 281-288 (2002).


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